PCR仪

相关疾病：
先天性卵巢发育不全综合征
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1 cctccgaaac catgaacttt ctgctctctt gggtgcactg gaccctggct ttactgctgt
61 acctccacca tgccaagtgg tgaagttcat ggacgtctac cagcgcagct attgccgtcc
121 aattgagacc ctggtggaca tcttccagga gtaccccgat gagatagagt atatcttcaa
181 gccgtcctgt gtgcccctaa tgcggtgtgc gggctgctgc aatgatgaag ccctggagtg
241 cgtgcccacg tcggagagca acgtcactat gcagatcatg cggatcaaac ctcaccaaag
301 ccagcacata ggagagatga gcttcctgca gcatagcaga tgtgaatgca gaccaaagaa
361 agatagaaca aagccagaaa atcactgtga gccttgttca gagcggagaa agcatttgtt
421 tgtccaagat ccgcagacgt gtaaatgttc ctgcaaaaac acagactcgc gttgcaaggc
481 gaggcagctt gagttaaacg aacgtacttg cagatgtgac aagccaaggc ggtgagccgg
541 g
版主:你好.以上是我的原文序列,麻烦你帮我设计一下引物好吗,谢谢!
我根据楼主的说明，把Tm值的范围改成了35-75，引物长度改为18-25，GG含量改为30-70，下面是结果
Primer Pair Data
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Primers for 1 (Entered)
These primers are for PCR
Forward-Primer CCTCCGAAACCATGAACTTTCT Reverse-Primer CCCGGCTCACCGCCTTGGCTTGT
Length: 22 Length: 23
Percent GC: 45 Percent GC: 69
Tm: 56 Tm: 72
Self-Anneal: 12 Self-Anneal: 16
End-Anneal: 4 End-Anneal: 8
Offset: 1 Offset: 541
Pair-Anneal: 14
Pair-End-Anneal: 8
Total valid primer pairs: 8
These primers will amplify a fragment of 541 basepairs
The sequence of DNA that will be amplified by these primers is
1 CCTCCGAAAC CATGAACTTT CTGCTCTCTT GGGTGCACTG GACCCTGGCT
51 TTACTGCTGT ACCTCCACCA TGCCAAGTGG TGAAGTTCAT GGACGTCTAC
101 CAGCGCAGCT ATTGCCGTCC AATTGAGACC CTGGTGGACA TCTTCCAGGA
151 GTACCCCGAT GAGATAGAGT ATATCTTCAA GCCGTCCTGT GTGCCCCTAA
201 TGCGGTGTGC GGGCTGCTGC AATGATGAAG CCCTGGAGTG CGTGCCCACG
251 TCGGAGAGCA ACGTCACTAT GCAGATCATG CGGATCAAAC CTCACCAAAG
301 CCAGCACATA GGAGAGATGA GCTTCCTGCA GCATAGCAGA TGTGAATGCA
351 GACCAAAGAA AGATAGAACA AAGCCAGAAA ATCACTGTGA GCCTTGTTCA
401 GAGCGGAGAA AGCATTTGTT TGTCCAAGAT CCGCAGACGT GTAAATGTTC
451 CTGCAAAAAC ACAGACTCGC GTTGCAAGGC GAGGCAGCTT GAGTTAAACG
501 AACGTACTTG CAGATGTGAC AAGCCAAGGC GGTGAGCCGG G
不知道可不可以，请指教！