小中大前辈
你用60-mm petri-dish.的阿,那么每次要用很多RANKL的阿,这个好贵啊。
你每一个well里面有多少ml呢?
谢谢
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I made sRANKL by myself so does not matter how big the cell culture vehicle used. However,if you purchase RANKL you should use 8-well chambered slides, of course depends on what is the purpose of your experiments. For instance, if you want to extract RNA from the cells, you have to use dishes otherwise you would not have enough products. If you want to see osteoclasteogenesis, I think either 8-well chambered slides or 48-well plate will do.
For 60-mm Petri-dishes, 4 ml medium/dish.
I know commercial RANKL is very expensive, around 180USD/10ug.