PCR仪

呵呵,我有个恒温检测SNPs的方法,可惜是公司商业秘密,个人觉得是检测技术里最好的,是相当的simple.

在HapMap之后，与SNP有关的另外一个需要密切关注的发展方向是Cancer Genome project: 美国NIH的Cancer Genome Atlas Project。

cuturl('http://cancergenome.nih.gov/index.asp')

The Cancer Genome Atlas (TCGA) is a comprehensive and coordinated effort to accelerate our understanding of the molecular basis of cancer through the application of genome analysis technologies, including large-scale genome sequencing.

通过对癌症病人的genome sequencing，希望发现相关的致病变异，其中主要包括SNP。Cancer Genome Atlas 的前期工作主要会集中对三种癌症的测序，包括 brain (glioblastoma), lung (squamous cell), and ovarian cancer.

相似在Breast Cancer和Colorectal Cancer的Genome Sequencing研究已经在去年的这篇Science文章上发表了, 不过不是Cancer Genome Atlas做的。

The figures below show the basic principles of the process when using Wild Type (WT) Primers.
The FIP and BIP are designed to contain a SNP nucleotide (in this case of Wild Type allele) at 5' end, respectively.
Using the WT primers, when the target gene is the WT allele, DNA synthesis from dumbbell-like starting structure proceeds and the LAMP amplification cycling continues. In contrast, when the target gene is the Mutant (MUT) allele, no DNA synthesis proceeds from dumbbell-like structure and the LAMP amplification cycling dose not occur. Even if DNA synthesis proceeds in one step due to miscopy, the amplification reaction is either halted in other steps or is delayed since repetition of this reaction continually checks at each cycling step of the DNA replication.

顺便说一下，我可不是这个公司的~~~只是对这个感兴趣~~

目前在帮师兄做SNP,但很多东西不清楚,看了你们的东西,受益匪浅.谢谢

楼上你说的那个做snp的准确性不是很好
做大规模筛查还可以，还有就是每次必须调整好
一次就做了，重复性也不好