PCR仪

因做RT-PCR,急需Wistar 大鼠Lmp7与Lmp2的引物.
如能提供,不胜感激.

我急需人VLDLR全长功能的PCR引物,请求高手帮帮忙.

1.物种： 关中奶山羊
2.基因名称：催乳素（PRL）
3.模板：cDNA
4.PCR类型及目的：RT-PCR
5.forward primer(5’-3’): 上游引物:5′ ACGAGAGCTTCCTGGTAATGAAGTGTGT 3′
reverse primer(5’-3’)：下游引物:5′ GGATGAATGTGGGCTTAGCAGTTGT 3′
6.扩增长度720bp
7.参考文献:无　

因为要做试验，急需兔的钾通道（Ito1、IK)RT-PCR引物序列，不知哪位能帮助我一下，在此谢谢！

做了半年做不出来，快被弄疯了，各位大虾帮帮忙，帮我看看这段序列设计个什么引物合适：
1 accaagggga aaatgaagtg gtgactcaaa tcatcgaaga ccctcgagat tacataggtc
61 cggaacctat ggccttcgtg accgacctcg agtcagagta gttcaataag gacctatcaa
121 gtttgggcaa tttttcgtcc ccgacacaaa aatgtcatcc gtgcttaaag catatgagcg
181 attcacgctc actcaagaac tgcaagatca gagtgaggaa ggtacaatcc cacctacaac
241 actaaaaccg gtaatcaggg tatttatact aacctctaat aacccagagc taagatcccg
301 gcttcttcta ttctgcctac ggattgttct cagtaatggt gcaagggatt cccatcgctt
361 tggagcatta ctcacaatgt tttcgctacc atcagccaca atgctcaatc atgtcaaatt
421 agctgaccag tcaccagaag ctgatatcga aagggtagag atcgatggct ttgaggaggg
481 atcattccgc ttaatcccca atgcacgttc aggtatgagc cgtggagaga tcaatgccta
541 tgctgcactt gcagaagatc tacctgacac actaaaccat gcaacacctt tcgttgattc
601 cgaagtcgag ggaactgcat gggatgagat tgagactttc ttagatatgt gttacagtgt
661 cctaatgcag gcatggatag tgacttgcaa gtgcatgact gcgccagacc aacctgctgc
721 ttctattgag aaacgcctgc aaaaatatcg tcagcaaggc aggatcaacc cgagatatct
781 cctgcaaccg gaggctcgac gaataatcca gaatgtaatc cggaagggaa tggtggtcag
841 acatttcctc acctttgaac tgcagcttgc ccgagcacaa agccttgtat caaataggta
901 ttatgctatg gtaggggatg ttggaaagta tatagagaat tgtggaatgg gaggcttctt
961 tttgacacta aaatatgcat taggaactag atggcccaca cttgctttag ctgcattttc
1021 aggagagcta acaaagctaa agtccctcat ggcattatac cagacccttg gtgagcaggc
1081 ccgatatttg gccctattgg agtcaccaca tttgatggat tttgctgcag caaactaccc
1141 actgctatat agctatgcta tgggaatagg ctatgtgtta gatgtcaaca tgaggaacta
1201 cgctttctcc agatcataca tgaacaagac atatttccaa ttgggaatgg aaactgcaag
1261 aaaacaacag ggtgcagttg acatgaggat ggcagaagat ctcggtctaa ctcaagccga
1321 acgcaccgag atggcaaata cacttgccaa attgaccaca gcaaatcgag gggcagacac
1381 caggggagga gtcaacccgt tctcatctgt cactgggaca actcaggtgc ccgctgcagc
1441 aacaggtgac acactcgaga gttacatggc agcggatcga ctgaggcaga gatatgctga
1501 tgcaggcacc catgatgatg agatgccacc attggaagag gaggaagagg acgacacatc
1561 tgcaggtcca cgcactggac caactcttga acaagtggcc ttggacatcc agaacgcagc
1621 agttggagct cccatccata cagatgacct gaatgccgca ctgggtgatc ttgacatcta
1681 gacaattcag atcccaatct aaaattgaca tacctaattg attagttaga tggaactaca
1741 gtggattcca taaggttcct gcctaccatc ggctttaaag aaaaaaatag gcccggacgg
1801 gttagcaaca agcgactgcc ggtgccaaca gcgcaatcca caatctacaa tggatcccac
1861 tgatctgagc ttctccccag atgagatcaa taagctcata gagacaggcc tgaatactgt
1921 agagtatttt acttcccaac aagtcacagg aacatcctct cttggaaaga atacaatacc
1981 accaggggtc acaggactac taaccaatgc tgcagaggca aagatccaag agtcaactaa
2041 ccatcagaag ggctcagttg gtgggggtgc aaaaccaaag aaaccgcgac caaaaattgc
2101 cattgtgcca gcagatgaca aaacagtgcc cggaaagccg atcccaaacc ctctattagg
2161 tctggactcc accccgagca cccaaactgt gcttgatcta agtgggaaaa cattaccatc
2221 aggatcctat aagggggtta agcttgcgaa atttggaaaa gaaaatctga tgacacggtt
2281 catcgaggaa cccagagaga atcctatcgc aaccagttcc cccatcgatt ttaagagggg
2341 cagggatacc ggcgggttcc atagaaggga gtactcaatc ggatgggtgg gagatgaagt
2401 caaggtcact gagtggtgca atccatcctg ttctccaatc accgctgcag caaggcgatt
2461 tgaatgcact tgtcaccagt gtccagtcac ttgctctgaa tgtgaacgag atacttaata
2521 cagtgagaaa tttggactct cggatgaatc aactggagac aaaagtagat cgcattctct
2581 catctcagtc tctaatccag accatcaaga atgacatagt tggacttaaa gcagggatgg
2641 ctactttaga aggaatgatt acaactgtga aaatcatgga cccgggagtt cccagtaatg
2701 ttactgtgga agatgtacgc aagacactaa gtaaccatgc tgttgttgtg ccagaatcat
2761 tcaatgatag tttcttgact caatctgaag atgtaatttc acttgatgag ttggctcgac
2821 caactgcaac aagtgttaag aagattgtca ggaaggttcc tcctcagaag gatctgactg
2881 gattgaagat tacactagag caattggcaa aggattgcat cagcaaaccg aagatgaggg
2941 aagagtatct cctcaaaatc aaccaggctt ccagtgaggc tcagctaatt gacctcaaga
3001 aagcaatcat ccgcagtgca atttgatcaa gaaacaccca attacactac actggtatga

3061 cactgtacta accctgaggg ttttagaaaa aacgattaac gataaataag cccgaacact
3121 acacactacc tgaggcagcc atgccatcca tcagcattcc cgcagacccc accaatccac
3181 gtcaatcaat aaaagcgttc ccaattgtga tcaacagtga tgggggtgag aaaggccgct
3241 tggttaaaca actacgcaca acctacttga atgacctaga tactcatgag ccactggtga
3301 cattcataaa tacctatgga ttcatctacg aacaggatcg ggggaatacc attgtcggag
3361 aggatcaact tgggaagaaa agagaggctg tgaccgctgc aatggttacc cttggatgtg
3421 ggcctaatct accatcatta gggaatgtcc tgggacaact gagggaattc caggtcactg
3481 ttaggaagac atccagcaaa gcggaagaga tggtctttga aattgttaag tatccgagaa
3541 tatttcgggg tcatacatta atccagaaag gactagtctg tgtctccgca gaaaaatttg
3601 ttaagtcacc agggaaaata caatctggaa tggactatct cttcattccg acatttctgt
3661 cagtgactta ctgtccagct gcaatcaaat ttcaggtacc tggccccatg ttgaaaatga
3721 gatcaagata cactcagagc ttacaacttg aactaatgat aagaatcctg tgtaagcccg
3781 attcgccact tatgaaggtc catacccctg acaaggaggg aagaggatgt cttgtatcag
3841 tatggctgca tgtatgcaac atcttcaaat caggaaacaa gaatggcagt gagtggcagg
3901 aatactggat gagaaagtgt gctaacatgc aacttgaagt gtcgattgca gatatgtggg
3961 gaccaactat cataattcat gccagaggtc acattcccaa aagtgctaag ttgttttttg
4021 gaaagggtgg atggagctgc catccacttc acgaagttgt tccaagtgtc actaaaacac
4081 tatggtccgt gggctgtgag attacaaagg cgaaggcaat aatacaagag agtagcatct
4141 ctcttctcgt ggagactact gacatcataa gtccaaaagt caaaatttca tctaagcatc
4201 gccgctttgt gaaatcaaat tggggtctgt tcaagaaaac taaatcactg cctaacctga
4261 cggagctgga atgactgacc tctaatcgag actacaccgc cgcaaactat aggtgggtgg
4321 tacctcagtg attaatcttg taagcactga tcgtaggcta caacacacta atattatcca
4381 gattagagag cttaattagc tctgtattaa taataacact actattccaa taactggaat
4441 caccagcttg atttatctcc aaaatgattc aaagaaaaca aatcatatta agactatcct
4501 aagcacgaac ccatatcgtc cttcaaatca tgggtactat aattcaattt ctggtggtct
4561 cctgtctatt ggcaggagca ggcagccttg atccagcagc cctcatgcaa atcggtgtca
4621 ttccaacaaa tgtccggcaa cttatgtatt atactgaggc ctcatcagca ttcattgttg
4681 tgaagttaat gcctacaatt gactcgccga ttagtggatg taatataaca tcaatttcaa
4741 gctataatgc aacagtgaca aaactcctac agccgatcgg tgagaatttg gagacgatta
4801 ggaaccagtt gattccaact cggaggagac gccggtttgc aggggtggtg attggattag
4861 ctgcattagg agtagctact gccgcacagg tcactgccgc agtggcacta gtaaaggcaa
4921 atgaaaatgc tgcggctata ctcaatctca aaaatgcaat ccaaaaaaca aatgcagcag
4981 ttgcagatgt ggtccaggcc acacaatcac taggaacggc agttcaagca gttcaagatc
5041 acataaacag tgtggtaagt ccagcaatta cagcagccaa ttgtaaggcc caagatgcta
5101 tcattggctc aatcctcaat ctctatttga ccgagttgac aaccatcttc cacaatcaaa
5161 ttacaaaccc tgcattgagt cccattacaa ttcaagcttt aaggatccta ctggggagta
5221 ccttgccgac tgtggtcgaa aaatctttca atacccagat aagtgcagct gagcttctct
5281 catcagggtt attgacaggc cagattgtgg gattagattt gacctatatg cagatggtca
5341 taaaaattga gctgccaact ttaactgtac aacctgcaac ccagatcata gatctggcca
5401 ccatttctgc attcattaac aatcaagaag tcatggccca attaccaaca cgtgttatgg
5461 tgactggcag cttgatccaa gcctatcccg catcgcaatg caccattaca cccaacactg
5521 tgtactgtag gtataatgat gcccaagtac tctcagatga tactatggct tgcctccaag
5581 gtaacttgac aagatgcacc ttctctccag tggttgggag ctttctcact cgattcgtgc
5641 tgttcgatgg aatagtttat gcaaattgca ggtcgatgtt gtgcaagtgc atgcaacctg
5701 ctgctgtgat cctacagccg agttcatccc ctgtaactgt cattgacatg tacaaatgtg
5761 tgagtctgca gcttgacaat ctcagattca ccatcactca attggccaat gtaacctaca
5821 atagcaccat caagcttgaa tcatcccaga tcttgtctat tgatccgttg gatatatccc
5881 aaaatctagc tgcggtgaat aagagtctaa gtgatgcact acaacactta gcacaaagtg
5941 acacatatct ttctgcaatc acatcagcta cgactacaag tgtattatcc ataatagcaa
6001 tctgtcttgg atcgttaggt ttaatattaa taatcttgct cagtgtagtt gtgtggaagt
6061 tattgaccat tgtcgttgct aatcgaaata gaatggagaa ttttgtttat cataaataag
6121 cattccacca ctcacgatct gatctcagtg agaaaaatca acctgcaact cttggaacaa
6181 gataagacag tcatccatta gtaattttta agaaaaaaac gataggaccg aacctagtat
6241 tgaaagaacc gtctcggtca atctaggtaa tcgagctgat accgtctcgg aaagctcaaa
6301 tcatgctgcc tgatccggaa gatccggaaa gcaagaaagc tacaaggaga gcaggaaacc
6361 taattatctg cttcctattc atcttctttc tgtttgtaac cttcattgtt ccaactctaa
6421 gacacttgct gtcctaacac ctgctatagg ctatccactg catcatctct cctgccatac
6481 ttcctactca catcatatct attttaaaga aaaaataggc ccgaacacta atcgtgccgg
6541 cagtgccact gcacacacaa cactacacat acaatacact acaatggttg cagaagatgc
6601 ccctgttagg gccacttgcc gagtattatt tcgaacaaca actttaatct ttctatgcac
6661 actactagca ttaagcatct ctatccttta tgagagttta ataacccaaa agcaaatcat
6721 gagccaagca ggctcaactg gatctaattc tggattagga agtatcactg atcttcttaa
6781 taatattctc tctgtcgcaa atcagattat atataactct gcagtcgctc tacctctaca
6841 attggacact cttgaatcaa cactccttac agccattaag tctcttcaaa ccagtgacaa
6901 gctagaacag aactgctcgt ggagtgctgc actgattaat gataatagat acattaatgg
6961 catcaatcag ttctattttt caattgctga gggtcgcaat ctgacacttg gcccacttct
7021 taatatgcct agtttcattc caactgccac gacaccagag ggctgcacca ggatcccatc
7081 attctcgctc actaagacac actggtgtta tacacacaat gttatcctga atggatgcca
7141 ggatcatgta tcctcaaatc aatttgtttc catgggaatc attgaaccca cttctgccgg
7201 gtttccattc tttcgaaccc taaagactct atatctcagc gatggggtca atcgtaagag
7261 ctgctctatc agtacagttc cggggggttg tatgatgtac tgttttgttt ctactcaacc
7321 agagagggat gactactttt ctgccgctcc tccagaacaa cgaattatta taatgtacta
7381 taatgataca atcgtggagc gcataattaa tccacccggg gtactagatg tatgggcaac
7441 attgaaccca ggaacaggaa gcggggtata ttatttaggt tgggtgctct ttccaatata
7501 tggcggcgtg attaaaggta cgagtttatg gaataatcaa gcaaataaat actttatccc
7561 ccagatggtt gctgctctct gctcacaaaa ccaggcaact caagtccaaa atgctaagtc
7621 atcatactat agcagctggt ttggcaatcg aatgattcag tctgggatcc tggcatgtcc
7681 tcttcgacag gatctaacca atgagtgttt agttctgccc ttttctaatg atcaggtgct
7741 tatgggtgct gaagggagat tatacatgta tggtgactcg gtgtattact atcaaagaag
7801 caatagttgg tggcctatga ccatgctgta taaggtaacc ataacattca ctaatggtca
7861 gccatctgct atatcagctc agaatgtgcc cacacagcag gtccctagac ctgggacagg
7921 agactgctct gcaaccaata gatgtcccgg tttttgcttg acaggagtgt atgccgatgc
7981 ctggttactg accaaccctt cgtctaccag tacatttgga tcagaagcaa ccttcactgg
8041 ttcttatctc aacacagcaa ctcagcgtat caatccgacg atgtatatcg cgaacaacac
8101 acagatcata agctcacagc aatttggatc aagcggtcaa gaagcagcat atggccacac
8161 aacctgtttt agggacacag gctctgttat ggtatactgt atctatatta ttgaattgtc
8221 ctcatctctc ttaggacaat ttcagattgt cccatttatc cgtcaggtga cactatccta
8281 aaggcagaag ccttcaggtc tgacccagcc aatcaaagca ttataccaga ccatggaatg
8341 cataccaaac attattgaca ctaatgacac acaaaattgg ttttaagaaa aaccaagaga
8401 acaataggcc agaatggctg ggtctcggga gatattactc cctgaagtcc atctcaattc
8461 accaattgta aagcataagc tatactatta cattctactt ggaaacctcc caaatgagat
8521 cgaccttgac gatttaggtc cattacataa tcaaaattgg aatcagatag cacatgaaga
8581 gtctaactta gctcaacgct tggtaaatgt aagaaatttt ctaattaccc acatccctga
8641 tcttagaaag ggccattggc aagagtatgt caatgtaata ctgtggccgc gaattcttcc
8701 cttgatcccg gattttaaaa tcaatgacca attgcctctg ctcaaaaatt gggacaagtt
8761 agttaaagaa tcatgttcag taatcaatgc aggtacttcc cagtgcattc agaatctcag
8821 ctatggactg acaggtcgtg ggaacctctt tacacgatca cgtgaactct ctggtgaccg

8881 cagggatatt gatcttaaga cagttgtggc agcatggcat gactcagact ggaaaagaat
8941 aagtgatttt tggattatga tcaaattcca gatgagacaa ttaattgtta ggcaaacaga
9001 tcataatgat tctgatttaa tcacgtatat cgaaaataga gaaggcataa tcatcataac
9061 ccctgaactg gtagcattat ttaacactga gaatcataca ctaacataca tgacctttga
9121 aattgtactg atggtttcag atatgtacga aggtcgtcac aacattttat cactatgcac
9181 agttagcact tacctgaatc ctctgaagaa aagaataaca tatttattga gccttgtaga
9241 taacttagct tttcagatag gtgatgctgt atataacata attgctttgc tagaatcctt
9301 tgtatatgca cagttgcaaa tgtcagatcc catcccagaa ctcagaggac aattccatgc
9361 attcgtatgt tctgagattc ttgatgcact aagaggaact aatagtttca cccaggatga
9421 attaagaact gtgacaacta atttgatatc cccattccaa gatctgaccc cagatcttac
9481 ggctgaattg ctctgtataa tgaggctttg gggacacccc atgctcactg ccagtcaagc
9541 tgcaggaaag gtacgcgagt ctatgtgtgc tggaaaagta ttagactttc ccaccattat
9601 gaaaacacta gcctttttcc atactattct gatcaatgga tacaggagga agcatcatgg
9661 agtatggcca cccttaaact taccgggtaa tgcttcaaag ggtctcacgg aacttatgaa
9721 tgacaatact gaaataagct atgaattcac acttaagcat tggaaggaag tctctcttat
9781 aaaattcaag aaatgttttg atgcagacgc aggtgaggaa ctcagtatat ttatgaaaga
9841 taaggcaatt agtgccccaa aacaagactg gatgagtgtg tttagaagaa gcctaatcaa
9901 acagcgccat cagcatcatc aggtccccct accaaatcca ttcaatcgac ggctgttgct
9961 aaactttctc ggagatgaca aattcgaccc gaatgtggag ctacagtatg taacatcagg
10021 tgagtatcta catgatgaca cgttttgtgc atcatattca ctaaaagaga aggaaattaa
10081 acctgatggt cgaatttttg caaagttgac taagagaatg agatcatgtc aagttatagc
10141 agaatctctt ttagcgaacc atgctgggaa gttaatgaaa gagaatggtg ttgtgatgaa
10201 tcagctatca ttaacaaaat cactattaac aatgagtcag attggaataa tatccgagaa
10261 agctagaaag tcaactcgag ataacataaa tcaacctggt ttccagaata tccagagaaa
10321 taaatcacat cactccaagc aagtcaatca gcgagatcca agtgatgact ttgaattggc
10381 agcatctttt ttaactactg atctcaaaaa atattgttta caatggaggt accagacaat
10441 tatcccattt gctcaatcat taaacagaat gtatggttat cctcatctct ttgagtggat
10501 tcacttacgg ctaatgcgta gtacacttta cgtgggggat cccttcaacc caccagcaga
10561 taccagtcaa tttgatctag ataaagtaat taatggagat atcttcattg tatcacccag
10621 aggtggaatt gaagggctgt gtcaaaaggc ttggacaatg atatctatcg ctgtgataat
10681 tctatctgcc acagagtctg gcacacgagt aatgagtatg gtgcagggag ataatcaagc
10741 aattgctgtc accacacgag taccaaggag cctgccgact cttgagaaaa agactattgc
10801 ttttagatct tgtaatctat tctttgagag gttaaaatgt aataattttg gattaggtca
10861 ccatttgaaa gaacaagaga ctatcattag ttctcacttc tttgtttata gcaagagaat
10921 attctatcag gggaggattc taacgcaagc cttaaaaaat gctagtaagc tctgcttgac
10981 agctgatgtc ctaggagaat gcacccaatc atcatgttct aatcttgcaa ctactgtcat
11041 gaggttaact gagaatggtg ttgaaaaaga tatctgtttc tacttgaata tctatatgac
11101 catcaaacag ctctcctatg atatcatctt ccctcaagtg tcaattcctg gagatcagat
11161 cacattagaa tacataaata atccacacct ggtatcacga ttggctcttt tgccatccca
11221 gttaggaggt ctaaactacc tgtcatgcag taggctgttc aatcgaaaca taggcgaccc
11281 ggtggtttcc gcagttgcag atcttaagag attaattaaa tcaggatgta tggattactg
11341 gatcctttat aacttattag ggagaaaacc gggaaacggc tcatgggcta ctttagcagc
11401 tgacccgtac tcaatcaata tagagtatca ataccctcca actacagctc ttaagaggca
11461 cacccaacaa gctctgatgg aactcagtac gaatccaatg ttacgtggca tattctctga
11521 caatgcacag gcagaagaaa ataaccttgc taggtttctc ctggataggg aggtgatctt
11581 tccgcgtgta gctcacatca tcattgagca aaccagtgtc gggaggagaa aacagattca
11641 aggatatttg gattcaacta gatcgataat gaggaaatca ctagaaatta agcccttatc
11701 caataggaag cttaatgaaa tactggatta caacatcaat tacctagctt acaatttggc
11761 attactcaag aatgctattg aacctccgac ttatttgaag gcaatgacac ttgaaacatg
11821 tagcatcgac attgcaagga acctccggaa gctctcctgg gccccactct tgggtgggag
11881 aaatcttgaa ggattagaga cgccagatcc cattgaaatt actgcaggag cattaattgt
11941 tggatcgggc tactgtgaac agtgtgctgc aggagacaat cgattcacat ggtttttctt
12001 gccatctggt atcgagatag gaggggatcc ccgtgataat cctcctatcc gtgtaccgta
12061 cattggctcc aggactgatg agaggagggt agcctcaatg gcatacatca ggggtgcctc
12121 gagtagctta aaagcagttc ttagactggc gggagtgtac atctgggcat tcggagatac
12181 tctggagaat tggatagatg cactggattt gtctcacact agagttaaca tcacacttga
12241 acagctgcaa tccctcaccc cacttccaac ctctgccaat ctaacccatc ggttggatga
12301 tggcacaact accctaaagt ttactcctgc gagctcttac accttttcaa gtttcactca
12361 tatatcaaat gatgagcaat acctgacaat taatgacaaa actgcagatt caaatataat
12421 ctaccaacag ttaatgatca ctggactcgg aatcttagaa acatggaata atcccccaat
12481 caatagaaca ttcgaagaat ctaccctaca tttgcacact ggtgcatcat gttgtgtccg
12541 acctgtggac tcctgcattc tctcagaagc attaacagtc aagccacata ttacagtacc
12601 gtacagcaat aaatttgtat ttgatgagga cccgctatct gaatatgaaa ctgcaaaact
12661 ggaatcgtta tcattccaag cccaattagg caacattgat gctgtagata tgacaggtaa
12721 attaacatta ttgtcccaat tcactgcaag gcagattatc aatgcaatca ctggactcga
12781 tgagtctgtc tctcttacta atgatgccat tgttgcatca gactatgtct ccaattggat
12841 tagtgaatgc atgtatacca aattagatga attatttatg tattgtgggt gggaactact
12901 attggaacta tcctatcaaa tgtattatct gagggtagtt gggtggagta atatagtgga
12961 ttattcttac atgatcttga gaagaatccc gggtgcagca ttaaacaatc tggcatctac
13021 attaagtcat ccaaaacttt tccgacgagc tatcaaccta gatatagttg cccccttaaa
13081 tgctcctcat tttgcatctc tggactacat caagatgagt gtggatgcaa tactctgggg
13141 ctgtaaaaga gtcatcaatg tgctctccaa tggaggggac ttagaattag ttgtgacatc

13201 tgaagatagc cttattctca gtgaccgatc catgaatctc attgcaagga aattaacttt
13261 attatcactg attcaccata atggtttgga actaccaaag attaaggggt tctctcctga
13321 tgagaagtgt ttcgctttga cagaattttt gaggaaagtg gtgaactcag ggttgagttc
13381 aatagagaac ctatcaaatt ttatgtacaa tgtggagaac ccacggcttg cagcattcgc
13441 cagcaacaat tactacctga ccagaaaatt attgaattca atacgagata ctgagtcggg
13501 tcaagtagca gtcacctcat attatgaatc attagaatat attgatagtc ttaagctaac
13561 cccacatgtg cctggcacct catgcattga ggatgatagt ctatgtacaa atgattacat
13621 aatctggatc atagagtcta atgcaaactt ggagaagtat ccaattccaa atagccctga
13681 ggatgattcc aatttccata actttaagtt gaatgctcca tcgcaccata ccttacgccc
13741 attagggttg tcatcaactg cttggtataa gggtataagc tgctgcaggt accttgagcg
13801 attaaagcta ccacaaggtg atcatttata tattgcagaa ggtagtggtg ccagtatgac
13861 aatcatagaa tacctattcc caggaagaaa gatatattac aattctttat ttagtagtgg
13921 tgacaatccc ccacaaagaa attatgcacc aatgcctact cagttcattg agagtgtccc
13981 atacaagctc tggcaagcac acacagatca atatcccgag atttttgagg acttcatccc
14041 tctatggaac ggaaacgccg ccatgactga cataggaatg acagcttgtg tagaattcat
14101 catcaatcga gtcggcccaa ggacttgcag tttagtacat gtagatttgg aatcaagtgc
14161 aagcttaaat caacaatgcc tgtcaaagcc gataattaat gctatcatca ctgctacaac
14221 tgttttgtgc cctcatgggg tgcttattct gaaatatagt tggttgccat ttactagatt
14281 tagtactttg atcactttct tatggtgcta ctttgagaga atcactgttc ttaggagcac
14341 atattctgat ccagctaatc atgaggttta tttaatttgt atccttgcca acaactttgc
14401 attccagact gtctcgcagg caacaggaat ggcgatgact ttaactgatc aagggtttac
14461 tttgatatca cctgaaagaa taaatcagta ttgggatggt cacttgaagc aagaacgtat
14521 cgtagcagaa gcaattgata aggtggttct aggagaaaat gctctattta attcgagtga
14581 taatgaatta attctcaaat gtggagggac accaaatgca cggaatctca tcgatatcga
14641 gccagtcgca actttcatag aatttgaaca attgatctgc acaatgttga caacccactt
14701 gaaggaaata attgatataa caaggtctgg aacccaggat tatgaaagtt tattactcac
14761 tccttacaat ttaggtcttc ttggtaaaat cagtacgata gtgagattat taacagaaag
14821 gattctaaat catactatca ggaattggtt gatcctccca ccttcgctcc ggatgatcgt
14881 gaagcaggac ttggaattcg gcatattcag gattacttcc atcctcaatt ctgatcggtt
14941 cctgaagctt tctccaaata ggaaatactt gattgcacaa ttaactgcag gctacattag
15001 gaaattgatt gagggggatt gcaatatcga tctaaccaga cctatccaaa agcaaatctg
15061 gaaagcatta ggttgtgtag tctattgtca cgatccaatg gatcaaaggg agtcaacaga
15121 gtttattgat ataaatatta atgaagaaat agaccgcggg atcgatggcg aggaaatcta
15181 aacatatcaa gaatcagaat tagtttaaga aaaaagaaga ggattaatct tggttttccc
15241 cttggt
做RT-PCR检测用，多谢了！

哪位高手赐教，急求大鼠的NF-kappa p65和ICAM 1引物序列