小中大
原理如下:
The assay takes advantage of an environmentally sensitive fluorescence dye, such as Sypro Orange, and follows its signal changes while the protein undergoes thermal unfolding. When Sypro Orange is added to a properly folded protein solution, it is exposed in an aqueous environment and its fluorescence signal quenched. As the temperature rises, the protein undergoes thermal unfolding and exposes its hydrophobic core region. Sypro Orange then binds to the hydrophobic regions and becomes unquenched. This will result in the increase of fluorescence signal of Sypro Orange.
