小中大
5.Nuclei were washed once with buffer A without 0.1% Triton X-100 and then lysed in 200 ul of buffer B (3 mM EDTA, 0.2 mM EGTA, 1 mM dithiothreitol, and protease inhibitor mixture). 在你们的步骤中,其中第五个步骤说是用BufferB lysed。不知道bufferB 中什么组分可以破核膜啊?还望指点一下啊!谢谢啦!
bigbang_0_0 wrote:
以下是我们实验室用到的方法,屡试不爽,相关成果发表在Molecular Cell上。
Chromatin Fractionation
1.about 3 × 106 cells were washed with PBS and resuspended in 200 ul of buffer A