小中大以下是一些RT-PCR的技术操作及相应文献参考：有意者可从中得到一些启示。
. cuturl('http://www.research.umbc.edu/~jwolf/m12.htm')(UMBC)
First strand synthesis and subsequent PCR amplification. Based on LTI protocol
. cuturl('http://www.nwfsc.noaa.gov/protocols/revtrpcr.html')(NWFSC)
Procedure for cDNA synthesis on dynabeads oligo(dT)25
. cuturl('http://gause.usuhs.mil/rt.html')(Cause's Lab)
Detailed protocol for RT and PCR
. cuturl('http://wheat.pw.usda.gov/homepage/lazo/methods/lazo/revtrpcr.html')(Lazo Lab)
First strand cDNA synthesis and PCR amplification
. cuturl('http://www.biol.rug.nl/lacto/protocols/superscript.html')
Procedure either for RT-PCR and primer extension. For primer extension, just add hot dATP
. cuturl('http://www.microbiology.med.umn.edu/immunology/cDNA_RT-PCR.html')(Immunology Resource)
CDNA synthesis from mRNA and subsequent PCR amplification
. cuturl('http://zapruder.pds.med.umich.edu/users/Frank/HepC/HepC.html')(Hans Popper)
This protocol is for the non-isotopic detection of hepatitis C RNA and albumin mRNA (as an internal control) from 4 micron sections of formalin-fixed, paraffin-embedded liver biopsies by RT-PCR. The procedure for RNA extraction from formalin fixed paraffin embedded section and PCR amplification is described.
