颗粒计数器

大家好，我合成的纳米颗粒不稳定，大慨有20min的稳定期。因此我想避免复杂的制样过程，而去直接表征样本溶液中的纳米颗粒。听说AFM可以直接表征溶液样本，是吗？谁有相关的经验可以告诉我下吗？

好像还不能做原位的吧。

AFM当然可以测溶液
不过测溶液难度很大，不仅仅对设备要求高，对使用者技术也要求高。
如果仅仅是为了表征一下形貌，不建议用AFM

你有做过吗，请问你是怎么做的？我是做生物的，不这些不懂，但是我的论文里需要一个我所用的纳米颗粒的电镜图。我合成的纳米颗粒不稳定，我担心制样，干化过程中会破坏颗粒。

液体的AFM表征，我自己没做过。隔壁实验室做Bio-AFM，见过1-2次。
目前的SPM设备不少都带着个液相测试的附属功能
但是如何把图做漂亮很花时间和精力
你要找得到愿意给你做的人，你就去做吧。

除非对仪器和体系都非常有经验，否则要在20min内抓出一张液相AFM的图几乎不可能，得到的结果也不一定真实。
你想知道纳米颗粒的什么性质呢？如果只是尺寸，建议用散射方法。

AFM制样很简单，超过100nm的颗粒用AFM观察有点难，一般看个石墨烯或者碳纳米管还是可以的

5 nM左右，就只要知道个大慨尺寸就可以了。主要是证明这个颗粒合成了。

这是原始文献中用来表征这种颗粒的AFM方法：
The sample of dsDNA or ssDNA (10 μl) was deposited onto a freshly cleaved mica surface (Ted
Pella, Inc.) and left to adsorb for 10 min. Then 10 mM MOPS (12.5 mM Mg2+) (500 μl) was
added to the liquid cell and the sample was scanned in a tapping mode using Agilent AFM
series 5500 (Agilent Technologies, USA) with 0.08 N/m force constant cantilever of a Silicon
nitride cantilevers with sharpened Pyramidal tip (OMCL-TR400PSA, Olympus, Atomic Force
F&E GmbH, Mannheim, Germany). After engagement the tapping amplitude set point was
typically 2.0 volts and the scan rates ranged from 1-2 Hz. During the liquid AFM imaging the
better imaging was in most cases obtained with minimal loading forces applied and optimized
feedback parameters. Several AFM images, all 512 × 512 pixels, were obtained from separate
locations across the mica surfaces to ensure reproducibility of the results. All the images were
analyzed by using Gwyddion 2.16 software.
我看了，貌似没什么制样过程。你们帮我研究一下，难度在哪里？我打算请公司或者高校的人帮我做。

这是原始文献中用来表征这种颗粒的AFM方法：
The sample of dsDNA or ssDNA (10 μl) was deposited onto a freshly cleaved mica surface (Ted
Pella, Inc.) and left to adsorb for 10 min. Then 10 mM MOPS (12.5 mM Mg2+) (500 μl) was
added to the liquid cell and the sample was scanned in a tapping mode using Agilent AFM
series 5500 (Agilent Technologies, USA) with 0.08 N/m force constant cantilever of a Silicon
nitride cantilevers with sharpened Pyramidal tip (OMCL-TR400PSA, Olympus, Atomic Force
F&E GmbH, Mannheim, Germany). After engagement the tapping amplitude set point was
typically 2.0 volts and the scan rates ranged from 1-2 Hz. During the liquid AFM imaging the
better imaging was in most cases obtained with minimal loading forces applied and optimized
feedback parameters. Several AFM images, all 512 × 512 pixels, were obtained from separate
locations across the mica surfaces to ensure reproducibility of the results. All the images were
analyzed by using Gwyddion 2.16 software.
我看了，貌似没什么制样过程。你们帮我研究一下，难度在哪里？我打算请公司或者高校的人帮我做。