小中大
Before add MTT to 96-well plate, you do need to remove supernate then add MTT.
If you want directly to add MTT with supernate, that is not so called MTT, it's another
kind of regaent so called MTX.
I strongly believe that OD should be used in NM570, other ODs are not correct.
If useing od 570nm and the reading should in 1.0 to 2.0, but some times depend on
that ELISA Reader's liner range. This has to be checked. The reading range out of
2, some times means we put too much cell in the well, so try to reduce the
concentration of your seeding cell.