小中大1、Methods Enzymol. 1993; 218: 413-9.
Reverse transcription of mRNA by Thermus aquaticus DNA polymerase followed by polymerase chain reaction amplification.
Jones MD.
2、Anal Biochem. 1990 Nov 1; 190(2): 292-6. Related Articles
Amplification, detection, and automated sequencing of gibbon interleukin-2 mRNA by Thermus aquaticus DNA polymerase reverse transcription and polymerase chain reaction.
Shaffer AL, Wojnar W, Nelson W.
Reverse transcription-polymerase chain reaction (RT-PCR) is a gene expression assay by which messenger RNA (mRNA) production can be measured. This technique involves three steps: isolation of RNA from cells or tissues, the creation of a DNA copy of the desired message (cDNA) by viral reverse transcriptase enzymes (RT), and amplification of this DNA segment by the polymerase chain reaction (PCR) for subsequent quantitation and analysis. Here we describe a one-enzyme, one-step method combining the RT and PCR steps of conventional RT-PCR by exploiting the recently documented RT properties of Taq polymerase, the thermostable enzyme used for PCR amplification of DNA. ......
