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这是一些比较常见的保存Oligo的问题，大家可以看看。
Q1. How should the oligo be stored?
Normally, the oligo should be stable at -20°C and could be stored at that temperature for more than a year. Though it is stable in solutions it would be degraded by contamination of nucleases. Therefore, we recommend that oligo be stored as dried. If you want to store oligo in solution, it is better to divide the whole solution into several tubes and store them separately. Oligos could be decomposed due to 'Freezing and Thawing Effect' when the oligo solutions are frozen and melted repeatedly. For storage of oligodeoxyribonucleotide (DNA), pH value should not be lowered to acidic condition. In acidic condition, DNA can be decomposed by depurination. On the other hand, the phosphodiester bond of oligoribonucleotides can be broken by hydrolysis in the basic condition.
Q2. How should the oligo be resuspended?
We recommend the oligos be dissolved with a buffer like TE (10 mM Tri-HCl, 1 mM EDTA, pH 8.0) instead of sterilized water itself for longer term storage. Once resuspended, oligos should be kept frozen at -20°C for longer use. Since oligos might not be easily dissolved in sterilized water the addition of NaOH would help to dissolve oligos in water.
Q3. If oligos were left at room temperature for more than a week, do they still work?
Once dried, oligos are supposed to have a tremendous stability. Even in solution, they are reasonably stable. Therefore, for the most of cases, without the contamination of materials which can cause decomposition of oligos, they should still work well, even if they were left at room temperature for more than a week.
Q4. Do I have to treat fluorescent dye modified oligos specially in storage and handling?
If exposed to light, fluorescent dye modified oligos are more fragile than unmodified oligos and their fluorescence intensity will decrease over time. To maintain their fluorescence efficiency, fluorescent dye modified oligos should be stored in the dark at -20°C. Since Cy3 and Cy5 can be decomposed at a pH above 9, Cy3 and Cy5 modified oligos
