小中大这次我用12well plate, 10^6cell/well, from D2,0.5ml培养液的那个well,有好多细胞dying, 我不知道还要不要继续这个well.
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if cells partially dying from D2, it is a good phenomenon (RANKL effectively). your cells dying of overgrew (D9), no good.
check either your cells or the qulity of your RANKL. (I guess something wrong in your cells)