【讨论帖】蛋白芯片制作与应用 - 经验共享

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标题:【讨论帖】蛋白芯片制作与应用

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发表于 2013-4-20 15:28 资料个人空间短消息加为好友

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简介

ACLARA BioSciences, Inc., (used to be called Soane Biosciences) Hayward, California (Plastic chips and microfluidic systems based on "Lab-On-A-Chip" microfluidics US Patent 5,750,015: "Method and device for moving molecules by the application of a plurality of electrical fields") Wins NIST ATP Award in "Tools for DNA Diagnostics" for Project: Multiplexed Sample Preparation Microsystem for DNA Diagnostics
Affymetrix, Inc., Santa Clara, California (The technology leader; manufactures the widely used GeneChip®arrays, including HIV, p450, p53, Rat Toxicology U34 arrays, etc.)
Alexion Pharmaceuticals Inc., New Haven, Connecticut
AlphaGene, Inc., Woburn, Massachusetts (full length cDNA FLEX? and MicroFLEX library construction; High Throughput Gene Expression Profiling; High Throughput DNA Sequencing; Bioinformatics)
Applied Precision, Inc., ssaquah, Washington. ArrayWoRx is a wide field light source based microarray scanner, combines limitless wavelength possibilites with automation and image processing software.
ArrayIt, Making DNA chip.
Axon Instruments, Inc., Foster City, California (GenePix 4000 Integrated Microarray Scanner and Analysis Software, simultaneously scans microarray slides at two wavelengths using a dual laser scanning system, displays images from two wavelengths and a ratio image as they are acquired in real time; US$50,000)
AxyS Pharmaceuticals, La Jolla, California: Wins NIST ATP Award in "Tools for DNA Diagnostics" Project: Liquid Array Technology Development
BioChip Technologies
bioDevice Partners, Cohasset, MA. Provides consulting services to the micorarraying community in the area of optics and instrumentation
BioDiscovery, Inc., Los Angeles, California (ImaGene?, special image processing and data extraction software)
Biomedical Photometrics, Inc., (MACROscope? for reading genetic microarrays, in collaboration with Canadian Genetic Microarray Consortium)
bioMerieux, in vitro diagnostics
BioRobotics Ltd., Comberton, Cambridge, UK (MicroGrid, for arraying oligonucleotides or cDNA clones on glass slides and plastic chips)
Brax, Cambridge, UK
Cadus Pharmaceutical Corp., Tarrytown, New York (yeast living chip)
Caliper Technologies Corp., Palo Alto, California: LabChips? based on microfluidics. Awarded $2 million contract by NIST to develop high-throughput DNA diagnostic platform. Project: Reference Laboratory LabChip? DNA Diagnostics System
Cartesian Technologies, Inc., Irvine, CA. PixSys PA Series: for Automated liquid handling system for creating high-density arrays for genomics research. Scan Array 3000: A Fluorescent Imaging System for microarray biochips.
Celera, Rockville, Maryland
Cellomics, Inc., Pittsburgh, Pennsylvania (ArrayScan?, cell-based "High Content Screening" (HCS) for drug discovery)
Cepheid Sunnyvale, California (microfluidics)
Ciphergen Biosystems, Palo Alto, California. ProteinChip? Arrays for the investigation of proteins on the femtomole scale directly from their "native" environments. Based on Surface-Enhanced Laser Desorption/Ionization (SELDI?).
Clinical Micro Sensors, Inc., Pasadena, California: DNA microchip-based medical diagnostics; detection of directly detect DNA via electron transfer. Wins NIST ATP Award in "Tools for DNA Diagnostics" Project: DNA Diagnostics for the Point of Care Using Electronic Nucleic Acid Detection
Clontech
Corning Science Products Division, Acton, MA provides the (Corning Microarray Technology) CMT-GAPS amino silane coated slides and CMT-Hybridization chamber.
Corvas International, Inc., (2D gel, proteomics)
CuraGen Corp., New Haven, Connecticut. GeneCalling? and Quantitative Expression Analysis (QEA?), CuraMode, CuraTox
diaDexus, LLC, Santa Clara, California. joint venture between SmithKline Beecham Corp. and Incyte Pharmaceuticals, Inc.. Specialized in using microarray technology for molecular diagnostics
DNAmicroarray.com. offers complete "made to order" high density DNA microarray synthesis and analysiss services. Prices, availability, and turnaround time seem impressive.
Gel biochip
Gene Logic, Inc., Columbia, Maryland (Flow-thru ChipTM: has hundreds of thousands of discrete microscopic channels that pass completely through it. Probe molecules are attached to the inner surface of these channels, and target molecules flow through the channels, coming into close proximity to the probes. This proximity facilitates hybridization. READS?, Restriction Enzyme Analysis of Differentially-expressed Sequences, for capturing and analyzing the overall gene expression profile of a given cell or tissue type to identify drug targets)
GeneData AG (Basel, Switzerland), analysis of genomics and proteomics data: GeneData WorkBench, GeneData Expressionist.
Genemachines Genomic Instrumentation Services, Inc., Menlo Park, California (OmniGrid, glass slides or nylon membranes, similar to Dr. Pat Brown's)

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发表于 2013-4-20 15:32 资料个人空间短消息加为好友

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General Scanning Inc., Watertown, Massachusetts (laser scanning and micropositioning, manufactures MicroArray Biochip Scanning System: ScanArrayTM). Now called GSI Lumonics
GeneScreen, Inc., The Genetics Profiling Company
GeneTrace Systems
Genetic Analysis Technology Consortium (GATC)
Genetic MicroSystems Inc., Woburn, Massachusetts (instrumentation for DNA microarray-based analysis)
Genetix Ltd., Christchurch, Dorset, UK (Q-Bot, Q-Pix)
Genome Systems Inc., St. Louis, MO, a wholly owned subsidiary of Incyte Pharmaceuticals, Inc., GDA: Gene Discovery Array
Genometrix Inc., The Woodlands, Texas (Bioscanner?, GeneView®, Universal Arrays?, Risk-Tox)
Genomic Solutions, Ann Arbor, Michigan (Flexys? modular robotic system, GeneTAC? and Genomic Integrator? array analysis products automates the imaging and analysis of gene microarrays.)
Genosys Biotechnologies, Inc., The Woodlands, Texas (Panorama?E. coli Gene Arrays, 4,290 genes per array)
GENSET, Paris, France (specialized in pharmacogenomics)
Genzyme Molecular Oncology (SAGE®: Serial Analysis of Gene Expression)
HP GeneArray Scanner (used by Affymetrix and others)
Hyseq Inc., Sunnyvale, California (Sequencing By Hybridization. HyX platform and Gene Discovery, HyGnostics, and HyChip? modules)
Illumina, Inc., San Diego, California. utilizes fiber optics, microfabrication, and advanced information processing to create arrays where 250,000 discrete sensors fit on a probe the diameter of the head of a pin.
I.M.A.G.E. Consortium: "Sharing resources to achieve a common goal - the discovery of all genes"
Imaging Research, Inc., St. Catharines, Ontario, Canada. The company writes software, develops detection technologies, and integrates systems for image analysis. Its PC-based ArrayVisionTMsystem has been widely used for rapid and automated analysis of genome arrays.
Incyte Pharmaceuticals, Inc., Palo Alto, California (GEM Microarrays, GeneJetTM array, LifeSeq® Database with estimated 100,000 genes, and LifeArray Microarray Software)
Intelligent Bio-Instruments, Cambridge, Massachusetts
JMAR's Precision Systems, Inc., Chatsworth, CA. Designer and manufacturer of UV exposure and mask aligner systems specifically designed for bio-chip manufacturers. Also produces custom micropositioning systems for micro-spotting equipment and high resolution dimensional metrology and defect inspection systems for quality assurance of bio-chips and DNA micro-arrays.
Labman AutomationLtd., North Yorkshire, TS9 5JY, UK (HDMS: Labman High-Density Microarray Spotter)
Lifecodes Corp., Stamford, Connecticut (Lifecodes MicroArray System: LMAS)
Micronics, Inc., Redmond, Washington. microfluidics based systems for application to clinical laboratory diagnostics: Microcytometer?, H-Filter?, T-Sensor?, and O.R.C.A. µFluidics.
Molecular Applications Group, Palo Alto, CA. Stingray? is integrated software and database products for gene expression, gene function, and gene sequence analysis from microarray data. It is integrated with and dependent upon the use of Affymetrix's GeneChip® system and its Expression Data Mining Tool (EDMT) software.
Molecular Dynamics, Inc., Sunnyvale, California (Storm® and FluorImager®
Molecular Tool, Inc., Baltimore, Maryland. Genetic Bit Analysis, GBA®, Genomatic?. Acquired by Orchid Biocomputer on September 14, 1998.
Nanogen, San Diego, California (Electronic Addressing, Concentration, and Hybridization)
NEN Life Science Products, Boston, MA (MICROMAX? Human cDNA Microarray System I for differential gene expression analysis)
Oncormed Inc., (acquired by Gene Logic in July, 1998) characterizes genes to establish their clinical relevancy and provides molecular profiling of patients for pharmacogenomic and therapeutic purposes
Orchid Biocomputer, Inc., Princeton, New Jersey (a Sarnoff company) microfluidic chips; applying microfabrication processes in glass, silicon, and other materials to create three dimensional structures. Contained within these devices are small capillary channels less than a millimeter wide. Wins NIST ATP Award in "Tools for DNA Diagnostics" Project: Polymerase Signaling Assay for DNA Variation Detection on Universal Processor Arrays It also has a Web site on single nucleotide polymorphisms (SNPs).
Packard Instrument Company, Meriden, Connecticut. (BioChip Arrayer)
Partek, Inc., St. Peters, Missouri. Provider of pattern recognition and data visualization software for science and engineering. Its Partek Pro 2000 system has been used by companies to analyze microarray gene expression data.
PE Applied Biosystems, Wins NIST ATP Award in "Tools for DNA Diagnostics" for project: Integrated, Micro-Sample Preparation System for Genetic Analysis
PharmaSeq, Inc., Monmouth Junction (near Princeton), NJ. Developer of microtransponder-based technology for DNA diagnostic assays. Wins NIST ATP Award in "Tools for DNA Diagnostics" for project: Multiplex DNA Diagnostic Assay Based on Microtransponders
Phase-1 Molecular Toxicology, Inc., Santa Fe, New Mexico. Molecular and high throughput toxicology using gene chips (Licensed from Xenometrix)
Proteome, Inc.

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发表于 2013-4-20 15:37 资料个人空间短消息加为好友

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Protogene Laboratories, Palo Alto, California (Surface tension array on glass substrate; "Printing" reagents using drop-on-demand technology)
Radius Biosciences, Medfield, Massachusetts
Research Genetics, Huntsville, Alabama (GeneFilter)
Rosetta Inpharmatics, Kirkland, Washington.
Scanalytics, Inc. , Fairfax, VA. Its MicroArray Suite enables researchers to acquire, visualize, process, and analyze gene expression microarray data. Developed by scientists at the NIH's National Human Genome Research Institute.
Sequana Therapeutics (merged with Arris Pharmaceutical to become AxyS Pharmaceuticals), La Jolla, California
Sequenom, Hamburg, Germany, and San Diego, California (DNA MassArray, BiomassPROBE, Biomass SIZE, BiomassSEQUENCE, BiomassSCAN, BiomassINDEX, and SpectroChip)
Silicon Genetics, Redwood City, California (GeneSpring workbench for analyzing experiments based upon genomic expression experiments)
Spotfire, Inc., Cambridge, Massachusetts. Offers advanced data visualization capabilities including the ability to perform gene cluster analysis and metabolic pathway mapping. The Spotfire Array Explorer is ideal for any researcher performing microarray analysis.
Synomics Ltd., Cambridge, UK (bioinformatics)
Synteni, Inc., Fremont, California (acquired by Incyte Pharmaceuticals, Inc. in January 1998) (UniGEM? Gene Expression Microarray)
TeleChem International, Sunnyvale, California (offers whole system parts: ChipMaker, SmartChips, ArrayIt, Hybridization Cassette, ScanArray 3000, ImaGene Quantification Software)
Virtek Vision International Inc. (Ontario, Canada) ChipReader? is a high-sensitivity laser confocal system for rapid imaging of the DNA microarrays.
Vysis, Inc., Downers Grove, Illinois (CGH-Comparative Genomic Hybridization)
Xanthon, Research Triangle Park, North Carolina. develops microplate based mini-arrays for high throughput screening of compounds for their effects on gene expression.
Xenometrix, Inc., Boulder, CO (Gene Profile Assay and bioinformatics for gene induction profile analysis; a demo is available)

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发表于 2013-4-20 15:38 资料个人空间短消息加为好友

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“功能基因组和生物芯片”重大科技专项
方向： “功能基因组和生物芯片”重大专项的实施对于推进我国生命科学和生物技术的发展发挥了重要的作用，为我国生物技术产业开辟了广阔的前景，奠定了我国生物芯片新产业的基础。

技术突破：在医药方面，已经验证并具有重要应用前景的功能基因56个。其中，14个新的单基因遗传病致病基因、16个用于肿瘤血清学诊断和分子分型的基因的发现和确证，将开创我国新型医学分子诊断技术和产业的新局面；18个潜在的药物靶标、15个肿瘤药物先导化合物的发现，将改变我国医药产业原始创新能力不足的被动局面。

在农业方面，拥有我国自主知识产权的分蘖基因、脆杆基因、抗旱基因、营养高效利用基因已经开始应用于我国农作物新品种的选育，为我国农业生产的发展，保障粮食安全发挥重要作用。

在传统产业改造方面，通过功能基因组研究提高了对发酵工业生产菌株的优选技术，选择的优良青霉素生产菌株和维生素C生产菌株已经应用于工业化生产，显著增加了生产效率，提高了我国在青霉素和维生素产业的国际竞争能力。

社会效益：目前，我国已经形成了北京、上海、西安、天津和南京5个生物芯片研发和产业化基地。200多项生物芯片和配套设备新产品研制成功，其中6项产品获得上市许可。到2005年底，生物芯片基地的产品和技术服务销售额达到2亿元。特别值得一提的是，我国自主研发的生物芯片技术和产品已经开始向美国等发达国家出口，技术转让和出口近7000万元，开始打破我国在生物技术高端仪器设备方面完全依赖进口的局面。生物芯片技术和产业的发展也对我国疾病诊断、药物研发、食品安全监测等技术和产业领域产生了良好的推动作用。改变了我国生物技术领域原始性创新的被动局面。到2005年底，共申请国内专利1021项，批准178项，申请国际专利202项，批准34项。30多个功能明确的新基因有望在医疗诊断、药物创新等方面得到应用。10个左右新基因已经开始进行作物品种改良的尝试。

全面提升了我国生命科学和生物技术的总体水平。到2005年底，共发表论文1709篇，其中在国际刊物上发表的论文1151篇，影响因子超过10的高水平论文62篇。影响因子在5—10的论文241篇。自本专项实施以来，已经在《NATURE》、《SCI鄄ENCE》和《CELL》国际刊物上发表论文21篇。

继2000年完成国际人类基因组测序（HUGO）1％任务之后，我国科学家又于2005年顺利完成了所承担了人类SNP（单核苷酸多态性）作图国际计划（HapMap）10％的任务，且国际公认我国科学家完成的质量是最好的；目前又发起并领导了“国际人类肝脏蛋白质组计划”（HLPP）。此外，在“十五”期间还独立完成了水稻、家蚕、血吸虫等重要物种的全基因组测序工作。

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发表于 2013-4-20 15:40 资料个人空间短消息加为好友

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相关疾病：
肿瘤
请问哪里有监测喉癌血清的蛋白质芯片？还有多肿瘤蛋白质芯片的概念，是不是就是能检测十二种肿瘤标志物的芯片/

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发表于 2013-4-20 15:41 资料个人空间短消息加为好友

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相关疾病：
肿瘤肺癌乳腺癌胃癌胰腺癌

===========================================================================================

请问哪里有监测喉癌血清的蛋白质芯片？还有多肿瘤蛋白质芯片的概念，是不是就是能检测十二种肿瘤标志物的芯片/

==============================================================================================================

1）目前尚未研制出可以检测喉癌的蛋白质芯片。
2）多肿瘤蛋白质芯片即C12，开发商为上海数康。具体品种为CEA、AFP、CA125、CA19-9、NSE、CA242、HCG、HGH、CA15-3、F-PSA、PSA、Ferritin。
3）以上芯片检测时阳性率超过55%的有肝癌、肺癌、乳腺癌、子宫癌、卵巢癌、胃癌、肠癌、胰腺癌，不包括喉癌。

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发表于 2013-4-20 15:41 资料个人空间短消息加为好友

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请楼主谈谈蛋白芯片能否在3－5年内应用于临床检验！

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发表于 2013-4-20 15:42 资料个人空间短消息加为好友

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QUOTE:

原帖由 8princess8 于 2013-4-20 15:41 发表 bbcodeurl('http://bbs.antpedia.com/images/common/back.gif', '%s')
请楼主谈谈蛋白芯片能否在3－5年内应用于临床检验！

相关疾病：
肿瘤沙眼结核感染疾病

目前国内的蛋白芯片已经批准的共有以下5种，其中多肿瘤检测芯片已经覆盖了很多3甲医院，解脲脲原体，人乳头瘤病毒，沙眼衣原体抗体蛋白芯片的市场前景也看好。

1: 多肿瘤标志物蛋白芯片检测系统( 保护期、 2013-12-16 )
2: 丙型肝炎病毒分片段抗体检测试剂盒(蛋白芯片)( -----、 ----- )
3: 幽门螺旋杆菌抗体蛋白芯片检测系统( 保护期、 2014-07-18 )
4: 解脲脲原体，人乳头瘤病毒，沙眼衣原体抗体蛋白芯片检测系统( 保护期、 2014-07-18 )
5: 结核分枝杆菌IgG抗体检测试剂盒(蛋白芯片)( 待定、 null )

未来还会有自身免疫检测芯片，肿瘤亚型分型芯片，术前检测芯片和感染性疾病检测芯片等会陆续申报并投入市场。

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发表于 2013-4-20 15:43 资料个人空间短消息加为好友

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老兄好强呀！！佩服！！
我是做相互作用的尤其是药物方面的，不知能否与您的蛋白芯片联系起来，特别是应用FACS技术呀！◎多谢指教

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发表于 2013-4-20 15:43 资料个人空间短消息加为好友

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QUOTE:

原帖由 one 于 2013-4-20 15:43 发表 bbcodeurl('http://bbs.antpedia.com/images/common/back.gif', '%s')

老兄好强呀！！佩服！！
我是做相互作用的尤其是药物方面的，不知能否与您的蛋白芯片联系起来，特别是应用FACS技术呀！◎多谢指教

蛋白芯片应用于药物研究是一个很好的方向，相信不久的将来就会有很好的实验结果出来。
以后碰到问题就随时写出来大家一块学习讨论，共同提高吧！

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