中华肿瘤杂志（CHINESE JOURNAL OF ONCOLOGY
2002 Vol.24 No.4 P.327-330）上面写道：
人乳腺癌细胞包括MCF-7、SK-BR-3、T47D、MDA-MB-435s和ZR-75 -30
而HBL-100为正常乳腺细胞
英文杂志中研究HBL-100最早的一篇文章如下：
Cell Tissue Res. 1982;227(3):563-8. Related Articles, Links
A cell line (HBL-100) established from human breast milk.
Gaffney EV.
A continuous cell line (HBL-100) was obtained from primary cultures of cells derived from an early lactation sample of human milk. There was no evidence of a breast lesion in the milk donor. Karyotype analysis showed that all metaphases contained human chromosomes including a large acrocentric marker chromosome. Both desmosomes and cytoplasmic tonofibrils were observed during early passage. HBL-100 cells exhibited several characteristics of transformation including the ability to form colonies in soft agar, an aneuploid chromosome complement, and continuous growth.
在 Eur J Cancer Clin Oncol. 1989 May;25(5):883-9.写道：
MCF-7 breast cancer and HBL-100 normal breast cell lines，并且HBL-100 breast milk derived cell line
在Exp Cell Res. 1985 Sep;160(1):83-94.中写道
HBL-100细胞为来源于乳汁的人表皮永生化细胞，下面为这篇文章的题目和摘要，你可以参考一下：
Epithelial HBL-100 cell line derived from milk of an apparently healthy woman harbours SV40 genetic information.
Caron de Fromentel C, Nardeux PC, Soussi T, Lavialle C, Estrade S, Carloni G, Chandrasekaran K, Cassingena R.
The epithelial HBL-100 cell line was established in vitro from milk of an apparently healthy woman. It exhibits characteristics of transformation from the very beginning and evolves during in vitro maintenance, until becoming tumorigenic in nude mice. This immortal cell line represents a useful model for studying the progression of human epithelial cells toward malignancy. In the course of our investigations we detected a 94K protein in HBL-100 cells obtained from four different sources. This protein is shown to be indistinguishable from the SV40 large T-antigen on the basis of: Recognition by polyclonal and different monoclonal antibodies. Partial peptide map analysis. Specific binding capacity to the SV40 DNA origin of replication. The presence of a tandemly integrated SV40 genome is demonstrated by Southern blotting. Successful rescue of SV40 DNA by fusion with permissive COS-7, but not CV-1 cells, indicates that the SV40 T-antigen from HBL-100 cells is defective in a function essential to the replication of the viral DNA. The possible origin of the SV40 genetic information that we have detected in HBL-100 cells and the implications of this finding on studies involving this cell line are discussed.
不过，在Biochim Biophys Acta. 2000 May 31;1485(2-3):129-44.又写道两种细胞都为乳腺癌细胞
我自己没有用到过HBL-100,只是培养过MCF-7
但是通过以上最早文献的查阅，我自己认为HBL-100应该是来源于人乳汁的一种永生化乳腺细胞，正常的和乳腺癌细胞都不是很准确。
不知楼主是否同意？大家一起讨论讨论！
最新回复
avi317 (2012-8-13 13:26:15)
cuturl('http://www.dxy.cn/bbs/post/view?bid=66&id=5207284&sty=1')
看一下这个，你会理解的
flyxx05 (2012-8-13 13:26:40)
QUOTE:
没看明白啊,,,,那个里面只有MCF-7 ,没有HBL-100啊????还是不明白....
viviwang1987 (2012-8-13 13:27:04)
HBL-100是正常乳腺细胞，MCF-7为人乳腺癌细胞。
flyxx05 (2012-8-13 13:27:26)
ROSE李 (2012-8-13 13:27:51)
中文文献说法不一，不知道是正常还是肿瘤～～,想想国人的科研
cuturl('http://www.baidu.com/baidu?word=HBL-100&tn=myie2d')
详细英文介绍：
HBL-100
cuturl('http://www.biotech.ist.unige.it/cldb/cl1571.html')
MCF-7
cuturl('http://www.biotech.ist.unige.it/cldb/cl3366.html')
学会google
hyuu (2012-8-13 13:28:19)
中华肿瘤杂志（CHINESE JOURNAL OF ONCOLOGY
2002 Vol.24 No.4 P.327-330）上面写道：
人乳腺癌细胞包括MCF-7、SK-BR-3、T47D、MDA-MB-435s和ZR-75 -30
而HBL-100为正常乳腺细胞
英文杂志中研究HBL-100最早的一篇文章如下：
Cell Tissue Res. 1982;227(3):563-8. Related Articles, Links
A cell line (HBL-100) established from human breast milk.
Gaffney EV.
A continuous cell line (HBL-100) was obtained from primary cultures of cells derived from an early lactation sample of human milk. There was no evidence of a breast lesion in the milk donor. Karyotype analysis showed that all metaphases contained human chromosomes including a large acrocentric marker chromosome. Both desmosomes and cytoplasmic tonofibrils were observed during early passage. HBL-100 cells exhibited several characteristics of transformation including the ability to form colonies in soft agar, an aneuploid chromosome complement, and continuous growth.
在 Eur J Cancer Clin Oncol. 1989 May;25(5):883-9.写道：
MCF-7 breast cancer and HBL-100 normal breast cell lines，并且HBL-100 breast milk derived cell line
在Exp Cell Res. 1985 Sep;160(1):83-94.中写道
HBL-100细胞为来源于乳汁的人表皮永生化细胞，下面为这篇文章的题目和摘要，你可以参考一下：
Epithelial HBL-100 cell line derived from milk of an apparently healthy woman harbours SV40 genetic information.
Caron de Fromentel C, Nardeux PC, Soussi T, Lavialle C, Estrade S, Carloni G, Chandrasekaran K, Cassingena R.
The epithelial HBL-100 cell line was established in vitro from milk of an apparently healthy woman. It exhibits characteristics of transformation from the very beginning and evolves during in vitro maintenance, until becoming tumorigenic in nude mice. This immortal cell line represents a useful model for studying the progression of human epithelial cells toward malignancy. In the course of our investigations we detected a 94K protein in HBL-100 cells obtained from four different sources. This protein is shown to be indistinguishable from the SV40 large T-antigen on the basis of: Recognition by polyclonal and different monoclonal antibodies. Partial peptide map analysis. Specific binding capacity to the SV40 DNA origin of replication. The presence of a tandemly integrated SV40 genome is demonstrated by Southern blotting. Successful rescue of SV40 DNA by fusion with permissive COS-7, but not CV-1 cells, indicates that the SV40 T-antigen from HBL-100 cells is defective in a function essential to the replication of the viral DNA. The possible origin of the SV40 genetic information that we have detected in HBL-100 cells and the implications of this finding on studies involving this cell line are discussed.
不过，在Biochim Biophys Acta. 2000 May 31;1485(2-3):129-44.又写道两种细胞都为乳腺癌细胞
我自己没有用到过HBL-100,只是培养过MCF-7
但是通过以上最早文献的查阅，我自己认为HBL-100应该是来源于人乳汁的一种永生化乳腺细胞，正常的和乳腺癌细胞都不是很准确。
不知楼主是否同意？大家一起讨论讨论！
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