Cell line CHO-K1
Cell type Chinese hamster ovary cells
DSMZ No ACC 110
Origin subclone from parental CHO cell line that was initiated from an ovary biopsy of an
adult Chinese hamster in 1957
References Puck et al., J. Exp. Med. 108: 945-953 (1958); Ham et al., Proc. Natl. Acad. Sci
USA 53: 288-293 (1965); Kao et al., Proc. Natl. Acad. Sci. USA 60: 1275-1281 (1968)
Depositor Dr. P. E. Bryant, University of St. Andrews, St. Andrews, Fife, UK
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DSMZ Cell Culture Data
Morphology adherent, fibroblastoid cells, may be induced to undergo additional differentiation
Medium 90% Ham's F12 + 10% FBS
Subculture split confluent cultures 1:3 to 1:10 using trypsin/EDTA; seed out about 105 -
106 cells in an 80 cm2 flask; cells grow up in 3-4 days to confluence
Incubation at 37 °C with 5% CO2
Doubling time doubling time of ca. 24 hours
Harvest about 10 x 106 cells/80 cm2 or 3 x 106 cells/25 cm2
Storage frozen with 70% medium, 20% FBS, 10% DMSO at about 2-4 x 106 cells/ampoule
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DSMZ Scientific Data
Mycoplasma negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Fingerprint same Hinf I-(gtg)5 DNA profile as CHO-DHFR
Species confirmed as hamster with IEF of NP, PEP B
Cytogenetics near diploid karyotype with 12% polyploidy; 22(20-23); exhibits distinctive CHO
karyology
Viruses ELISA: reverse transcriptase negative
Cell line CHO-K1
Cell type Chinese hamster ovary cells
DSMZ No ACC 110
Origin subclone from parental CHO cell line that was initiated from an ovary biopsy of an
adult Chinese hamster in 1957
References Puck et al., J. Exp. Med. 108: 945-953 (1958); Ham et al., Proc. Natl. Acad. Sci
USA 53: 288-293 (1965); Kao et al., Proc. Natl. Acad. Sci. USA 60: 1275-1281 (1968)
Depositor Dr. P. E. Bryant, University of St. Andrews, St. Andrews, Fife, UK
--------------------------------------------------------------------------------
DSMZ Cell Culture Data
Morphology adherent, fibroblastoid cells, may be induced to undergo additional differentiation
Medium 90% Ham's F12 + 10% FBS
Subculture split confluent cultures 1:3 to 1:10 using trypsin/EDTA; seed out about 105 -
106 cells in an 80 cm2 flask; cells grow up in 3-4 days to confluence
Incubation at 37 °C with 5% CO2
Doubling time doubling time of ca. 24 hours
Harvest about 10 x 106 cells/80 cm2 or 3 x 106 cells/25 cm2
Storage frozen with 70% medium, 20% FBS, 10% DMSO at about 2-4 x 106 cells/ampoule
--------------------------------------------------------------------------------
最新回复
KGZ564 (2012-10-25 15:53:00)
CHO 指的是中国仓鼠卵巢细胞。不知你问哪一种是什么意思？
loli (2012-10-25 15:53:22)
Cell line CHO-K1
Cell type Chinese hamster ovary cells
DSMZ No ACC 110
Origin subclone from parental CHO cell line that was initiated from an ovary biopsy of an
adult Chinese hamster in 1957
References Puck et al., J. Exp. Med. 108: 945-953 (1958); Ham et al., Proc. Natl. Acad. Sci
USA 53: 288-293 (1965); Kao et al., Proc. Natl. Acad. Sci. USA 60: 1275-1281 (1968)
Depositor Dr. P. E. Bryant, University of St. Andrews, St. Andrews, Fife, UK
--------------------------------------------------------------------------------
DSMZ Cell Culture Data
Morphology adherent, fibroblastoid cells, may be induced to undergo additional differentiation
Medium 90% Ham's F12 + 10% FBS
Subculture split confluent cultures 1:3 to 1:10 using trypsin/EDTA; seed out about 105 -
106 cells in an 80 cm2 flask; cells grow up in 3-4 days to confluence
Incubation at 37 °C with 5% CO2
Doubling time doubling time of ca. 24 hours
Harvest about 10 x 106 cells/80 cm2 or 3 x 106 cells/25 cm2
Storage frozen with 70% medium, 20% FBS, 10% DMSO at about 2-4 x 106 cells/ampoule
--------------------------------------------------------------------------------
DSMZ Scientific Data
Mycoplasma negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Fingerprint same Hinf I-(gtg)5 DNA profile as CHO-DHFR
Species confirmed as hamster with IEF of NP, PEP B
Cytogenetics near diploid karyotype with 12% polyploidy; 22(20-23); exhibits distinctive CHO
karyology
Viruses ELISA: reverse transcriptase negative
张先生 (2012-10-25 15:53:56)
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CHO也分很多型啊，只是平时都没人注意罢了，详情参考ATCC
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张先生 (2012-10-25 15:54:16)
Cell line CHO-K1
Cell type Chinese hamster ovary cells
DSMZ No ACC 110
Origin subclone from parental CHO cell line that was initiated from an ovary biopsy of an
adult Chinese hamster in 1957
References Puck et al., J. Exp. Med. 108: 945-953 (1958); Ham et al., Proc. Natl. Acad. Sci
USA 53: 288-293 (1965); Kao et al., Proc. Natl. Acad. Sci. USA 60: 1275-1281 (1968)
Depositor Dr. P. E. Bryant, University of St. Andrews, St. Andrews, Fife, UK
--------------------------------------------------------------------------------
DSMZ Cell Culture Data
Morphology adherent, fibroblastoid cells, may be induced to undergo additional differentiation
Medium 90% Ham's F12 + 10% FBS
Subculture split confluent cultures 1:3 to 1:10 using trypsin/EDTA; seed out about 105 -
106 cells in an 80 cm2 flask; cells grow up in 3-4 days to confluence
Incubation at 37 °C with 5% CO2
Doubling time doubling time of ca. 24 hours
Harvest about 10 x 106 cells/80 cm2 or 3 x 106 cells/25 cm2
Storage frozen with 70% medium, 20% FBS, 10% DMSO at about 2-4 x 106 cells/ampoule
--------------------------------------------------------------------------------
我们平时最常用的是CHO-k1吗？
loli (2012-10-25 15:54:43)
yes it is CHO-k1
loli (2012-10-25 16:00:24)
看你做实验要干什么，你没看到下面对各个细胞系的注释里有讲吗：CHO-K1是来经原代细胞克隆后得到的，而其它的几种都是经质粒改造过的，高表达某种抗体。
一般如果做细胞效应的实验都是用CHO-K1系，其它的需针对你实验目的不同而选择。
自己没搞明白还说别人，哼！那不是叫种，是称为亚系了，受不了你！
TAT (2012-10-25 16:00:58)
自己没搞明白还说别人，哼！那不是叫种，是称为亚系了，受不了你！
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一般实验室里最常用的是CHO-K1吗？能肯定吗？之所以这么问，因为我去中科院细胞所买细胞的时候，他们告诉我普通CHO(即最常用的CHO)不是CHO-K1，CHO-K1比普通CHO贵的多，但他也不知道普通CHO究竟是哪一种？故有此问啊
另：我什么时候说你了？晕，哈哈，不好意思啦，欢迎继续讨论啊，我确实不是很懂啊，向MM学习！
【求助】这还真是个问题，晕