Normally, the oligo should be stable at -20°C and could be stored at that temperature for more than a year. Though it is stable in solutions it would be degraded by contamination of nucleases. Therefore, we recommend that oligo be stored as dried. If you want to store oligo in solution, it is better to divide the whole solution into several tubes and store them separately. Oligos could be decomposed due to 'Freezing and Thawing Effect' when the oligo solutions are frozen and melted repeatedly. For storage of oligodeoxyribonucleotide (DNA), pH value should not be lowered to acidic condition. In acidic condition, DNA can be decomposed by depurination. On the other hand, the phosphodiester bond of oligoribonucleotides can be broken by hydrolysis in the basic condition.
Q2. How should the oligo be resuspended?
We recommend the oligos be dissolved with a buffer like TE (10 mM Tri-HCl, 1 mM EDTA, pH 8.0) instead of sterilized water itself for longer term storage. Once resuspended, oligos should be kept frozen at -20°C for longer use. Since oligos might not be easily dissolved in sterilized water the addition of NaOH would help to dissolve oligos in water.
Q3. If oligos were left at room temperature for more than a week, do they still work?
Once dried, oligos are supposed to have a tremendous stability. Even in solution, they are reasonably stable. Therefore, for the most of cases, without the contamination of materials which can cause decomposition of oligos, they should still work well, even if they were left at room temperature for more than a week.
Q4. Do I have to treat fluorescent dye modified oligos specially in storage and handling?
If exposed to light, fluorescent dye modified oligos are more fragile than unmodified oligos and their fluorescence intensity will decrease over time. To maintain their fluorescence efficiency, fluorescent dye modified oligos should be stored in the dark at -20°C. Since Cy3 and Cy5 can be decomposed at a pH above 9, Cy3 and Cy5 modified oligos
丸子妹 (2011-9-24 12:25:17)
这里Sigma关于Oligo的指南,可以作为参考。
Oligos require certain basic storage and handling techniques in order to ensure trouble-free experiments. Proper storage of your oligo will maximize its shelf-life, allowing you to get the most use from your oligo. These simple dilution and weight/volume unit conversion guidelines will enable you to work easily with your oligo in your application.
All Genosys oligonucleotides are provided dry and ready for use upon resuspension. We recommend resuspending your oligos in a weak buffer, such as TE buffer (10 mM Tris, pH 7.5-8.0, 1 mM EDTA). Moreover, concentrated stock solutions are best kept in a weak buffer to ensure that the pH remains between 7 and 8. If TE buffer is not suitable for your application, Milli-Q™ grade water can be used. However, high grade water is usually slightly acidic and is not recommended for long term storage. DEPC-treated water may be used, but residual DEPC (diethyl pyrocarbonate) may inhibit subsequent enzymatic reactions. If desired, working solutions can be diluted from the stock solution using sterile, nuclease-free water.
Fluorescently-labeled oligonucleotides should be resuspended in TE buffer. If using fluo-rescently-modified oligonucleotides in your applications, it is important to note that the intensity of a fluorescent signal can often be impacted by the pH of the solution.Therefore, a buffered solution is recommended for resuspension for most fluorescently-labeled oligos.
For long-term storage of oligo stocks, we recommend that you aliquot, dry down the
sample and store desiccated at -20°C. Oligos modified with fluorescent dyes should be kept away from light as much as possible. Alkaline phosphatase modified oligonucleotides are shipped in 50% glycerol solution and can be stored between 4°C and -20°C, but no lower than -20°C.
最新回复
麻瓜 (2011-9-24 12:23:02)
丸子妹 (2011-9-24 12:23:27)
如果要更长期的保存,最好是干粉。
丸子妹 (2011-9-24 12:23:43)
Q1. How should the oligo be stored?
Normally, the oligo should be stable at -20°C and could be stored at that temperature for more than a year. Though it is stable in solutions it would be degraded by contamination of nucleases. Therefore, we recommend that oligo be stored as dried. If you want to store oligo in solution, it is better to divide the whole solution into several tubes and store them separately. Oligos could be decomposed due to 'Freezing and Thawing Effect' when the oligo solutions are frozen and melted repeatedly. For storage of oligodeoxyribonucleotide (DNA), pH value should not be lowered to acidic condition. In acidic condition, DNA can be decomposed by depurination. On the other hand, the phosphodiester bond of oligoribonucleotides can be broken by hydrolysis in the basic condition.
Q2. How should the oligo be resuspended?
We recommend the oligos be dissolved with a buffer like TE (10 mM Tri-HCl, 1 mM EDTA, pH 8.0) instead of sterilized water itself for longer term storage. Once resuspended, oligos should be kept frozen at -20°C for longer use. Since oligos might not be easily dissolved in sterilized water the addition of NaOH would help to dissolve oligos in water.
Q3. If oligos were left at room temperature for more than a week, do they still work?
Once dried, oligos are supposed to have a tremendous stability. Even in solution, they are reasonably stable. Therefore, for the most of cases, without the contamination of materials which can cause decomposition of oligos, they should still work well, even if they were left at room temperature for more than a week.
Q4. Do I have to treat fluorescent dye modified oligos specially in storage and handling?
If exposed to light, fluorescent dye modified oligos are more fragile than unmodified oligos and their fluorescence intensity will decrease over time. To maintain their fluorescence efficiency, fluorescent dye modified oligos should be stored in the dark at -20°C. Since Cy3 and Cy5 can be decomposed at a pH above 9, Cy3 and Cy5 modified oligos
丸子妹 (2011-9-24 12:25:17)
Oligos require certain basic storage and handling techniques in order to ensure trouble-free experiments. Proper storage of your oligo will maximize its shelf-life, allowing you to get the most use from your oligo. These simple dilution and weight/volume unit conversion guidelines will enable you to work easily with your oligo in your application.
All Genosys oligonucleotides are provided dry and ready for use upon resuspension. We recommend resuspending your oligos in a weak buffer, such as TE buffer (10 mM Tris, pH 7.5-8.0, 1 mM EDTA). Moreover, concentrated stock solutions are best kept in a weak buffer to ensure that the pH remains between 7 and 8. If TE buffer is not suitable for your application, Milli-Q™ grade water can be used. However, high grade water is usually slightly acidic and is not recommended for long term storage. DEPC-treated water may be used, but residual DEPC (diethyl pyrocarbonate) may inhibit subsequent enzymatic reactions. If desired, working solutions can be diluted from the stock solution using sterile, nuclease-free water.
Fluorescently-labeled oligonucleotides should be resuspended in TE buffer. If using fluo-rescently-modified oligonucleotides in your applications, it is important to note that the intensity of a fluorescent signal can often be impacted by the pH of the solution.Therefore, a buffered solution is recommended for resuspension for most fluorescently-labeled oligos.
For long-term storage of oligo stocks, we recommend that you aliquot, dry down the
sample and store desiccated at -20°C. Oligos modified with fluorescent dyes should be kept away from light as much as possible. Alkaline phosphatase modified oligonucleotides are shipped in 50% glycerol solution and can be stored between 4°C and -20°C, but no lower than -20°C.
饭团团 (2011-9-24 12:25:44)
自己摸索,怎样好就怎样做得了。
冷太阳 (2011-9-24 12:26:03)
笔笔 (2011-9-24 12:26:34)
丸子妹 (2011-9-24 12:27:05)
如果要更长期的保存,最好是干粉。
xiexie !-----
大猫 (2011-9-24 12:27:27)
大猫 (2011-9-24 12:27:46)
几×的??
我用的是5mmol的,0.5×的。
稀释引物时大家用什么?