查看完整版本请点击这里：
【求助】ESI质谱，试着解析了一下
用反相柱制备了一个生物寡肽，拿去打ESI质谱，试着解析了一下，是否正确，请有经验的大神指教！！谢谢！【求助】ESI质谱，试着解析了一下
发现有些问题无法解释：相对丰度最高的那个m/z 375.2239，是什么东东？同样地还有其它一些无法解释的峰：
m/z263.1964、m/z430.9138、m/z516.3134等等。
查看完整版本请点击这里：
【求助】ESI质谱，试着解析了一下
【求助】ESI质谱，试着解析了一下
58672973.snap.jpg
最新回复
zouyou (2015-12-16 15:02:55)
今生如此 (2015-12-16 15:03:24)
132.1020 0.00
158.9640 0.00
182.0820 0.00
204.0630 0.00
220.0375 0.00
263.1964 0.00
282.2791 0.00
313.1762 0.00
338.3414 0.00
360.3238 0.00
375.2239 0.00
413.3742 0.00
430.9138 0.00
469.4364 0.00
516.3134 0.00
569.3038 0.00
598.2940 0.00
641.5948 0.00
675.6773 0.00
702.8634 0.00
838.8387 0.00
-----------------------------------
然后分析结果如下：
6 extracted sequence tags at a precision of 0.200 Da
3 Met Pro Arg
132.10 263.20 360.32 516.31
-0.054 -0.075 0.112
3 Met Arg Glu
182.08 313.18 469.44 598.29
-0.054 -0.159 0.185
3 Arg Met Glu
182.08 338.34 469.44 598.29
-0.158 -0.055 0.185
2 Arg Arg
204.06 360.32 516.31
-0.160 0.112
2 Met Arg
282.28 413.37 569.30
-0.055 0.172
1 Tyr
675.68 838.84
-0.098
Leu and Ile are isobaric. Only Leu reported.
Legend:
- Sequence tag
- Mass values
- Mass deviations (in Da)
只有前面3中有可能，从第一个M/Z峰看，后面3种应该不是。从紫外图谱看，有275吸收，可能含有苯环，排除第1种，只剩下第2（YMRE）、3(YRME)种了。但两种都有出峰，还证明不了究竟是哪个啊？？？
难道不纯？？？
huali (2015-12-16 15:03:46)
所用质谱类型？校正精度？
母离子分子量是多少？
青青草 (2015-12-16 15:04:10)
钻石 (2015-12-16 15:04:46)
TITLE=query=16824
PEPMASS=567.82983
CHARGE=2+
SCANS=10018
RTINSECONDS=3884.346
169.053 30.369642
171.249 5.8928757
172.216 15.733374
172.923 17.814219
175.122 903.8535
176.482 108.077286
177.248 150.83904
183.156 99.45152
185.066 40.313503
187.189 38.15329
189.12 35.622673
190.06 34.00462
191.98 60.301144
193.118 36.400223
200.954 75.16594
203.965 15.209862
205.097 3291.6628
209.256 8.593015
211.273 45.225563
212.455 73.84433
213.241 16.567816
218.055 121.54267
219.274 32.017136
221.432 98.79402
224.971 10.549514
229.305 7.8825245
233.089 8.156205
236.28 97.45488
239.12 16.950783
239.916 209.68634
241.316 62.361755
243.105 17.308548
252.386 32.814693
253.076 16.74273
255.123 23.190811
258.109 170.23505
259.14 3194.1104
261.261 27.102144
262.427 105.14769
263.219 239.85858
264.003 70.65573
264.694 11.672335
266.103 28.817064
267.825 55.29625
270.259 157.96957
271.143 654.86743
275.321 82.3887
276.191 126.14318
280.143 22.529055
284.093 57.557533
286.291 20.34434
287.289 51.68265
288.228 2734.644
292.269 42.226673
295.991 47.90095
297.028 338.00995
299.102 170.79541
302.98 29.061007
304.228 2917.529
306.393 68.16261
307.121 30.446507
308.269 22.87841
310.246 10.5646925
313.165 72.664505
314.309 156.72917
318.418 17.94422
322.224 42.957764
322.981 15.226016
324.424 62.796787
325.184 63.47594
326.099 43.740322
327.449 88.26248
333.718 45.058556
335.023 23.473227
337.186 12.658334
339.022 11.100794
340.866 29.217354
342.925 41.38197
348.33 81.030624
350.625 12.83403
354.818 16.17976
356.247 39.549137
359.235 648.8535
360.027 167.12672
361.118 110.722466
362.286 290.7038
366.076 70.551025
367.302 9.845418
368.153 56.52041
372.171 203.14867
373.362 120.124054
374.375 434.0631
375.461 7.871987
377.341 249.30486
381.471 21.082546
384.451 36.912647
385.2 25.214085
386.069 92.1264
387.189 1712.1931
389.274 209.55635
392.249 42.044273
393.396 35.54155
396.145 171.58936
397.394 28.158028
398.618 106.97371
400.046 72.732864
401.336 4261.664
403.521 44.2925
405.241 2257.9453
406.335 83.349625
407.657 102.259026
411.039 23.648188
419.685 201.43863
421.341 67.88757
425.126 36.283337
425.935 169.417
427.2 12.545986
431.071 34.576538
433.231 59.194504
435.799 45.369244
436.51 22.302963
437.248 11.971346
438.396 34.05881
440.262 131.96564
442.088 107.50722
443.157 295.97552
444.293 283.62598
449.603 25.475079
451.778 43.841824
453.046 342.59387
454.235 86.79257
457.123 2587.7578
459.243 137.01862
460.214 159.31897
461.304 374.56097
465.972 802.1713
466.622 92.63174
467.52 161.40137
469.33 106.1501
470.084 1146.6746
471.02 55.88342
472.272 135.27197
475.339 49.553493
476.755 184.93042
478.393 634.7117
480.847 186.5759
483.081 78.35956
485.514 1182.0212
486.206 43.64317
487.68 106.373535
488.344 1673.419
489.27 151.0896
492.283 133.61938
494.226 32.728752
498.591 53.14993
499.514 129.32626
504.006 13.4634905
505.548 192.11337
506.332 1634.6294
507.092 162.69827
509.599 688.6417
512.245 33.096043
516.363 11033.493
517.508 941.6533
520.286 322.72144
525.726 12.552803
528.439 134.24628
531.17 34.07973
531.788 114.97632
532.757 156.64594
537.205 1683.604
539.607 89.70396
540.184 15.236631
541.125 1191.323
545.525 57.82872
546.068 124.90318
550.018 19324.04
552.17 71.807655
553.325 57.906933
557.571 145.02423
558.996 416.8162
567.445 18.768856
583.223 723.6338
584.123 329.8946
591.448 340.85825
595.443 103.40437
597.924 61.657772
599.172 62.32579
600.116 76.75032
601.284 2760.5652
613.554 65.56621
615.318 57.058456
619.305 1398.2361
620.518 200.0625
627.584 4.568072
629.448 14057.302
630.941 37.491302
635.399 17.225233
641.633 5.215313
652.238 43.71095
657.389 11.883195
659.107 20.56044
664.52 71.65474
665.344 20.079643
669.194 53.304073
670.713 7.447984
672.174 13.333517
678.671 30.508888
680.73 6.5501375
682.509 95.371826
683.75 60.672413
686.438 9.355066
689.15 86.0953
691.562 365.61383
692.595 44.30387
698.056 429.6672
699.249 37.79284
712.379 118.00801
712.703 469.31055
714.432 143.52058
716.2 379.2999
716.769 191.74586
717.473 21.210402
718.615 72.50905
719.992 22.549654
725.447 223.01106
728.403 65.944565
730.537 13634.867
731.588 1911.7227
734.219 901.6926
736.756 59.459435
739.389 21.201746
740.401 80.563065
744.641 170.81174
747.557 66.347565
751.583 65.46155
755.969 22.818472
【求助】ESI质谱，试着解析了一下