[求助]细胞株提供

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• 麦丽素1986 (2012-2-15 12:54:03)

  我要的是CYP3A4-Caco-2 Cells等转基因细胞
  
  CYP3A4、CYP1A2、CYP2C9等转基因细胞系资料不对呀可不可以再帮查一下

• 西子 (2012-2-15 12:54:42)

  CACO－2 ：
  
  ATCC Number:HTB-37
  
  Designation:Caco-2 Depositors:J Fogh
  
  Biosafety Level: 1 Shipped:frozen
  
  Medium &
  Serum:See Propagation Growth
  Properties:adherent
  
  Organism:Homo sapiens (human) Morphology:epithelial
  
  
  Tissue:colon; colorectal adenocarcinoma
  Permits/Forms:
  
  In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
  
  Related Cell Culture Products
  
  
  Comments:This line was isolated from a primary colonic tumor.
  Upon reaching confluence, the cells express characteristics of enterocytic differentiation.
  Caco-2 cells express retinoic acid binding protein I and retinol binding protein II and are keratin positive.
  The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.
  Receptors:heat stable enterotoxin (Sta, E. coli); epidermal growth factor (EGF)
  Virus Resist:human immunodeficiency virus (HIV, LAV)
  Tumorigenic:Yes, in nude mice; forms moderately well differentiated adenocarcinoma consistent with colonic primary (grade II)
  Antigen
  Expression:Blood Type O; Rh+
  Cytogenetic Analysis:The stemline modal chromosome number is 96, occurring at 16% with polyploidy at 3.2%. Ten common markers were detected i.e., t(1q;?), 10q-, t(11q17q) and 7 others. The t(1q17q) and M11 were found in a portion of cells. The ins(2), 10q-, and t(15q;?) were generally paired, and t(11q;17q) and t(21q;?) were mostly three-copied. Normal N9 was absent, and N21 was lost in some cells. One to 4 small acrocentric chromosomes were detected. No Y chromosome with bright distal q-band was detected by Q-observation.
  Isoenzymes:AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 1; Me-2, 1; PGM1, 1; PGM3, 1
  Age:72 years
  Gender:male
  Ethnicity:Caucasian
  Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 80%; fetal bovine serum, 20%
  Temperature: 37.0 C
  Subculturingrotocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Maintain cultures at a cell concentration between 1 X 10 exp4 and 1 X 10 exp5 cell/cm2.
  Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
  Medium Renewal: 1 to 2 times per week
  Freeze
  Medium:Complete growth medium, 95%; DMSO, 5%
  Storage temperature: liquid nitrogen vapor temperature
  Doubling Time:about 20 hours
  Related
  Products:Recommended medium (without the additional supplements or serum described under ATCC Medium) - ATCC 30-2003
  recommended serum - ATCC 30-2020
  derivative - ATCC CRL-2102
  References:18385: Didier ES , et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. J. Eukaryot. Microbiol. 43: 34-43, 1996. PubMed: 8563708
  22409: Jumarie C , Malo C . Caco-2 cells cultured in serum-free medium as a model for the study of enterocytic differentiation in vitro. J. Cell. Physiol. 149: 24-33, 1991. PubMed: 1939345
  22536: Fogh J , et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
  22539: Fogh J , et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
  22564: Adachi A , et al. Productive, persistent infection of human colorectal cell lines with human immunodeficiency virus. J. Virol. 61: 209-213, 1987. PubMed: 3640832
  22861: Trainer DL , et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874
  23009: Cohen MB , et al. Receptors for Escherichia coli heat stable enterotoxin in human intestine and in a human intestinal cell line (Caco-2). J. Cell. Physiol. 156: 138-144, 1993. PubMed: 8100232
  23012: Basson MD , et al. Effect of tyrosine kinase inhibition on basal and epidermal growth factor-stimulated human Caco-2 enterocyte sheet migration and proliferation. J. Cell. Physiol. 160: 491-501, 1994. PubMed: 8077287
  23118: Heinen CD , et al. Microsatellite instability in colorectal adenocarcinoma cell lines that have full-length adenomatous polyposis coli protein. Cancer Res. 55: 4797-4799, 1995. PubMed: 7585508
  23148: Gilbert T , Rodriguez-Boulan E . Induction of vacuolar apical compartments in the Caco-2 intestinal epithelial cell line. J. Cell Sci. 100: 451-458, 1991. PubMed: 1808199
  23191: Rigothier MC , et al. A new in vitro model of Entamoeba histolytica adhesion, using the human colon carcinoma cell line Caco-2: scanning electron microscopic study. Infect. Immun. 59: 4142-4146, 1991. PubMed: 1937772
  23416: Levin MS . Cellular retinol-binding proteins are determinants of retinol uptake and metabolism in stably transfected Caco-2 cells. J. Biol. Chem. 268: 8267-8276, 1993. PubMed: 8463337
  23563: Trotter PJ , Storch J . Fatty acid esterification during differentiation of the human intestinal cell line Caco-2. J. Biol. Chem. 268: 10017-10023, 1993. PubMed: 8387510
  25093: Santoro IM , Groden J . Alternative splicing of the APC gene and its association with terminal differentiation. Cancer Res. 57: 488-494, 1997. PubMed: 9012479
  32376: White LJ , et al. Attachment and entry of recombinant norwalk virus capsids to cultured human and animal cell lines. J. Virol. 70: 6589-6597, 1996. PubMed: 8794293
  32562: Baier LJ , et al. A polymorphism in the human intestinal fatty acid binding protein alters fatty acid transport across Caco-2 cells. J. Biol. Chem. 271: 10892-10896, 1996. PubMed: 8631905
  32794: Kutchera W , et al. Protaglandin H synthase 2 is expressed abnormally in human colon cancer: evidence for a transcriptional effect. Proc. Natl. Acad. Sci. USA 93: 4816-4820, 1996. PubMed: 8643486
  33127: Grindstaff KK , et al. Translational regulation of Na,K-ATPase alpha1 and beta1 polypeptide expression in epithelial cells. J. Biol. Chem. 271: 23211-23221, 1996. PubMed: 8798517

• u234 (2012-2-15 12:55:12)

  
  我要的是CYP3A4等转基因细胞
  转基因
  细胞
  转基因

• nn255 (2012-2-15 12:56:23)

  现在代理ATCC细胞株的国内公司很多。你们的到货周期？价格？多少的美金比价？如何处理投诉？

• 爱老虎游tt (2012-2-15 12:56:54)

  到货安全周期大概为4-6周，可没有像“adychen “所说的20天那么快，呵呵。
  价格方面要看您具体订购什么产品，金额多大，公司对客户有多项优惠制度。
  如果产品在运输途中出现死亡的现象，我公司无偿为您订购相同株。
  谢谢支持！

• duoduo (2012-2-15 12:57:20)

  
  我想请问你有没有正常人角质形成细胞系。可不可以发一些相关资料给我，我想购这些细胞　。如果订购的话大概要多长时间能够到货? 价格多少?