【求助】我的小肽只有3.5KD,这种低分子量的marker到哪里?

问了几家公司,在网上也搜了搜,没发现低分子量的marker,向各位求助!
我也来说两句 查看全部回复

最新回复

  • DONT (2014-6-08 10:48:04)


    这个行不?别忘了用Tris-Tricine的胶,因为你的分子量太小了。
    New Polypeptide Standards:
    SDS-PAGE and Kaleidoscope
    Bio-Rad’s new polypeptide standards are available as
    SDS-PAGE molecular weight standards or as prestained
    Kaleidoscope standards. This new class of standards provides
    valuable information specifically for research involving
    polypeptide and small molecular weight proteins.
    The SDS-PAGE molecular weight standards are specifically
    designed for determining the molecular weights of peptides
    and small proteins on SDS-PAGE gels. Each tube contains six
    proteins, ranging from 1,423 to 26,625 daltons, which create
    a distinctive pattern of even intensity bands when separated on
    a 16.5% Tris-Tricine or 10–20% Tris-Tricine gel. The standards
    produce excellent results when stained with Coomassie®
    G-250 dye.
    The Kaleidoscope Polypeptide Standards contain five individually
    colored proteins for easy identification of the protein
    bands. The Kaleidoscope standards are useful for assessing the
    transfer efficiency of a Western blot, and for monitoring an
    electrophoretic gel during the run. The different colors make
    band identification very easy and serve as a quick reference to
    estimating the molecular weight range.
    The molecular weights of prestained standards vary from one
    lot to another because the amount of dye that binds to a protein
    molecule is variable. The molecular weight range of the five
    polypeptide standards is from 3,496 to 31,000 daltons. Each
    lot of Kaleidoscope Polypeptide Standards is calibrated using
    the SDS-PAGE polypeptide molecular weight standards. A calibration
    card is included with every lot of the Kaleidoscope
    Polypeptide Standards.
    Every batch of Polypeptide SDS-PAGE Standards is tested for
    proper mobility to insure consistent quality and reliable control
    for gel-to-gel variability. Kaleidoscope Polypeptide Standards
    are also held to rigorous quality standards to ensure lot to lot
    consistency in band intensity and migration.
    SDS-PAGE and Kaleidoscope Polypeptide Standards are provided
    pre-blended in a stable storage buffer which allows the
    standards to be stored at -20 °C for up to 1 year without
    freeze/thaw degradation, eliminating the need to aliquot. Each
    vial is shipped with instructions and is guaranteed for 1 year
    when stored at -20 °C.


    65515160.jpg

  • DONT (2014-6-08 10:48:27)


    Specification


    23197942.jpg

  • DONT (2014-6-08 10:48:44)

    Oder Information.


    82423651.jpg

  • 49888 (2014-6-08 10:49:15)

    我前段时间也在找低分子量Marker,上海东风生化技术有限公司有最低至2000-3000的分子量Mark出售。你打他们的电话联系吧 02162211157或02128722708
  • bamboo16 (2014-6-08 10:49:55)


    SIGMA公司有货号C6210
    供应商联系方式
    公司名称 公司网址 联系人 联系方式
    北京舒伯伟化工仪器有限责任公司 cuturl('www.super-chem.com') 沈雪明 54673506
    吉泰科技有限公司 cuturl('www.genetimes.com.cn') 姜伟 54262677
    基因有限公司 cuturl('www.genehk.com') 鲁晓岩 64951899
    上海化学试剂公司 cuturl('www.reagent.com.cn') 周欣 63219357
    BIO-RAD cuturl('www.bio-rad.com') 金昌峰 64260808
    晶美生物工程公司 cuturl('www.jingmei.com') 何晓晨 64664434
    东方进出口公司 cuturl('www.osic-sh.com.cn') 王柯 64746540
    上海普飞生物技术有限公司 cuturl('www.pufei.com') 黄涛 51154951
    上海华运 cuturl('www.gilson.com.cn') 顾勤 34243614
           
    厂商联系方式
    Sigma-Aldrich中国有限公司 cuturl('www.sigma-aldrich.com')   63862766
    Invitrogen上海办事处 cuturl('www.invitrogen.com.cn') 64481068
     
    Merck上海贸易公司 cuturl('www.merck-china.com')   32224788
    Eppendorf上海代表处 cuturl('www.eppendorf.cn')   68760880
    Hyclone上海办事处 cuturl('www.hyclone.com.cn')   8008100242
    Promega上海公司 cuturl('www.shpromega.com.cn')   64820450
  • bamboo16 (2014-6-08 10:55:22)


    赛白盛也有一种:2-20kd的。
    cuturl('http://www.sbsbio.com/pro8_1.asp')
    还有:
    cuturl('http://www.neb-china.com/cn/products/proteintool/P7702.htm')
  • qumm1985 (2014-6-08 10:55:43)


    广州威佳公司有,条带大小分别是3KD.5KD,7KD,14KD,20KD,150块钱每管,可用20次左右,效果很好,我们实验室一直都用它,叫做多肽marker
  • qumm1985 (2014-6-08 10:56:04)

    谢谢各位的热心帮助,是不是价格很贵呀?